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Starch-Chitosan Polyplexes: A Versatile Carrier System for Anti-Infectives and Gene Delivery

Yasar, H, Duy-Khiet, H, De Rossi, C, Herrmann, J, Gordon, S, Loretz, B and Lehr, C-M (2018) Starch-Chitosan Polyplexes: A Versatile Carrier System for Anti-Infectives and Gene Delivery. Polymers, 10 (3). ISSN 2073-4360

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Abstract

Despite the enormous potential of nanomedicine, the search for materials from renewable resources that balance bio-medical requirements and engineering aspects is still challenging. This study proposes an easy method to make nanoparticles composed of oxidized starch and chitosan, both isolated from natural biopolymers. The careful adjustment of C/N ratio, polymer concentration and molecular weight allowed for tuning of particle characteristics. The system’s carrier capability was assessed both for anti-infectives and for nucleic acid. Higher starch content polyplexes were found to be suitable for high encapsulation efficiency of cationic anti-infectives and preserving their bactericidal function. A cationic carrier was obtained by coating the anionic polyplex with chitosan. Coating allowed for a minimal amount of cationic polymer to be employed and facilitated plasmid DNA loading both within the particle core and on the surface. Transfection studies showed encouraging result, approximately 5% of A549 cells with reporter gene expression. In summary, starch-chitosan complexes are suitable carriers with promising perspectives for pharmaceutical use.

Item Type: Article
Uncontrolled Keywords: Science & Technology; Physical Sciences; Polymer Science; polymeric nanoparticles; renewable polysaccharides; anionic starch; cationic anti-infectives; transfection; DRUG-DELIVERY; POLYMERIC NANOPARTICLES; BIODEGRADABLE NANOPARTICLES; PHARMACEUTICAL APPLICATIONS; INHALED ANTIBIOTICS; PROTEIN DELIVERY; SURFACE-CHARGE; RELEASE; CYTOTOXICITY; FUNCTIONALIZATION
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: MDPI
Related URLs:
Date Deposited: 11 Mar 2019 09:02
Last Modified: 11 Mar 2019 09:04
DOI or Identification number: 10.3390/polym10030252
URI: http://researchonline.ljmu.ac.uk/id/eprint/10277

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