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A Novel, All-Optical Tool for Controllable and Non- Destructive Poration of Cells with Single-Micron Resolution

Casey, DR and Wylie, D and Gallo, J and Dent, MR and Salehi-Reyhani, A and Wilson, R and Brooks, NJ and Long, NJ and Willison, KR and Klug, DR and Neil, MAA and Neale, SL and Cooper, JM and Ces, O (2015) A Novel, All-Optical Tool for Controllable and Non- Destructive Poration of Cells with Single-Micron Resolution. In: Optics in the Life Sciences . (Optics in the Life Sciences, 12th- 15th April 2015, Vancouver, BC).

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Abstract

We demonstrate controllable poration within ≈1 µm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience. As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single- and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell’s plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Cell analysis; Thin films
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry
Divisions: Built Environment
Publisher: Optical Society of America
Related URLs:
Date Deposited: 03 Jun 2015 08:46
Last Modified: 13 Apr 2016 23:50
DOI or Identification number: 10.1364/BODA.2015.BW1A.5
URI: http://researchonline.ljmu.ac.uk/id/eprint/1128

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