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Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding.

Solari, V and Rudd, TR and Guimond, SE and Powell, AK and Turnbull, JE and Yates, EA (2015) Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding. Organic and Biomolecular Chemistry, 13. pp. 6066-6072. ISSN 1477-0539

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Abstract

Phage display antibodies are widely used to follow heparan sulfate (HS) expression in tissues and cells. We demonstrate by ELISA, that cations alter phage display antibody binding profiles to HS and this is mediated by changes in polysaccharide conformation, demonstrated by circular dichroism spectroscopy. Native HS structures, expressed on the cell surfaces of neuroblastoma and fibroblast cells, also exhibited altered antibody binding profiles following exposure to low mM concentrations of these cations. Phage display antibodies recognise conformationally-defined HS epitopes, rather than sequence alone, as has been assumed, and resemble proteins in being sensitive to changes in both charge distribution and conformation following binding of cations to HS polysaccharides.

Item Type: Article
Uncontrolled Keywords: 0304 Medicinal And Biomolecular Chemistry, 0305 Organic Chemistry, 1115 Pharmacology And Pharmaceutical Sciences
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Royal Society of Chemistry
Date Deposited: 22 May 2015 12:27
Last Modified: 28 Apr 2016 23:50
URI: http://researchonline.ljmu.ac.uk/id/eprint/1145

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