Kunda, NK and Alfagih, IM and Dennison, SR and Somavarapu, S and Merchant, Z and Hutcheon, GA and Saleem, IY (2015) Dry powder pulmonary delivery of cationic PGA-co-PDL nanoparticles with surface adsorbed model protein. International journal of pharmaceutics, 492 (1-2). pp. 213-222. ISSN 1873-3476
Dry powder pulmonary delivery of cationic PGA-co-PDL nanoparticles with surface adsorbed model protein_corrected proof.pdf - Accepted Version
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Pulmonary delivery of macromolecules has been the focus of attention as an alternate route of delivery with benefits such as; large surface area, thin alveolar epithelium, rapid absorption and extensive vasculature. In this study, a model protein, bovine serum albumin (BSA) was adsorbed onto cationic PGA-co-PDL polymeric nanoparticles (NPs) prepared by a single emulsion solvent evaporation method using a cationic surfactant didodecyldimethylammonium bromide (DMAB) at 2% w/w (particle size: 128.64±06.01nm and zeta-potential: +42.32±02.70mV). The optimum cationic NPs were then surface adsorbed with BSA, NP:BSA (100:4) ratio yielded 10.01±1.19μg of BSA per mg of NPs. The BSA adsorbed NPs (5mg/ml) were then spray-dried in an aqueous suspension of L-leucine (7.5mg/ml, corresponding to a ratio of 1:1.5/NP:l-leu) using a Büchi-290 mini-spray dryer to produce nanocomposite microparticles (NCMPs) containing cationic NPs. The aerosol properties showed a fine particle fraction (FPF, dae<4.46μm) of 70.67±4.07% and mass median aerodynamic diameter (MMAD) of 2.80±0.21μm suggesting a deposition in the respiratory bronchiolar region of the lungs.The cell viability was 75.76±03.55% (A549 cell line) at 156.25μg/ml concentration after 24h exposure. SDS-PAGE and circular dichroism (CD) confirmed that the primary and secondary structure of the released BSA was maintained. Moreover, the released BSA showed 78.76±1.54% relative esterolytic activity compared to standard BSA.
|Uncontrolled Keywords:||1115 Pharmacology And Pharmaceutical Sciences|
|Subjects:||R Medicine > RS Pharmacy and materia medica|
|Divisions:||Pharmacy & Biomolecular Sciences|
|Date Deposited:||21 Aug 2015 11:08|
|Last Modified:||15 Aug 2016 23:50|
|DOI or Identification number:||10.1016/j.ijpharm.2015.07.015|
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