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The serum opsonin L-ficolin is detected in lungs of human transplant recipients following fungal infections and modulates inflammation and killing of Aspergillus fumigatus.

Bidula, S and Sexton, DW and Abdolrasouli, A and Shah, A and Reed, A and Armstrong-James, D and Schelenz, S (2015) The serum opsonin L-ficolin is detected in lungs of human transplant recipients following fungal infections and modulates inflammation and killing of Aspergillus fumigatus. Journal of Infectious Diseases, 212 (2). pp. 234-246. ISSN 1537-6613

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L-ficolin, is detected in human lungs of transplant patients following fungal infections and modulates inflammation and killing of A. fumigatus 2015 accepted version (1).pdf - Accepted Version

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Abstract

BACKGROUND: Invasive aspergillosis (IA) is a life-threatening systemic fungal infection in immunocompromised individuals that is caused by Aspergillus fumigatus. The human serum opsonin, L-ficolin, has been observed to recognize A. fumigatus and could participate in fungal defense. METHODS: Using lung epithelial cells, primary human monocyte-derived macrophages (MDMs), and neutrophils from healthy donors, we assessed phagocytosis and killing of L-ficolin-opsonized live A. fumigatus conidia by flow cytometry and microscopy. Additionally, cytokines were measured by cytometric bead array, and L-ficolin was measured in bronchoalveolar lavage (BAL) fluid from lung transplant recipients by enzyme-linked immunosorbent assay. RESULTS: L-ficolin opsonization increased conidial uptake and enhanced killing of A. fumigatus by MDMs and neutrophils. Opsonization was also shown to manifest an increase in interleukin 8 release from A549 lung epithelial cells but decreased interleukin 1β, interleukin 6, interleukin 8, interleukin 10, and tumor necrosis factor α release from MDMs and neutrophils 24 hours after infection. The concentration of L-ficolin in BAL fluid from patients with fungal infection was significantly higher than that for control subjects (P = .00087), and receiving operating characteristic curve analysis highlighted the diagnostic potential of L-ficolin for lung infection (area under the curve, 0.842; P < .0001). CONCLUSIONS: L-ficolin modulates the immune response to A. fumigatus. Additionally, for the first time, L-ficolin has been demonstrated to be present in human lungs.

Item Type: Article
Additional Information: his is a pre-copyedited, author-produced PDF of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record J Infect Dis. (2015) 212 (2): 234-246. is available online at: http://dx.doi.org/10.1093/infdis/jiv027
Uncontrolled Keywords: 11 Medical And Health Sciences, 06 Biological Sciences
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Oxford University Press
Related URLs:
Date Deposited: 05 Nov 2015 10:55
Last Modified: 22 Jan 2016 00:50
DOI or Identification number: 10.1093/infdis/jiv027
URI: http://researchonline.ljmu.ac.uk/id/eprint/2012

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