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High prevalence of trypanosomes in European badgers detected using ITS-PCR.

Ideozu, EJ and Whiteoak, AM and Tomlinson, AJ and Robertson, A and Delahay, RJ and Hide, G (2015) High prevalence of trypanosomes in European badgers detected using ITS-PCR. Parasites and Vectors, 8 (1). pp. 1-6. ISSN 1756-3305

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Abstract

BACKGROUND: Wildlife can be important sources and reservoirs for pathogens. Trypanosome infections are common in many mammalian species, and are pathogenic in some. Molecular detection tools were used to measure trypanosome prevalence in a well-studied population of wild European badgers (Meles meles). FINDINGS: A nested ITS-PCR system, that targeted the ribosomal RNA gene locus, has been widely used to detect pathogenic human and animal trypanosomes in domestic animals in Africa and some wildlife hosts. Samples from a long-term DEFRA funded capture-mark-recapture study of wild badgers at Woodchester Park (Gloucestershire, SW England) were investigated for trypanosome prevalence. A total of 82 badger blood samples were examined by nested ITS-PCR. Twenty-nine of the samples were found to be positive for trypanosomes giving a prevalence of 35.4 % (25.9 % - 46.2 %; 95 % CI). Infection was not found to be linked to badger condition, sex or age. Analysis of DNA sequence data showed the badgers to be infected with Trypanosoma (Megatrypanum) pestanai and phylogenetic analysis showed the Woodchester badger trypanosomes and T. pestanai to cluster in the Megatrypanum clade. CONCLUSIONS: The results show that the ITS Nested PCR is an effective tool for diagnosing trypanosome infection in badgers and suggests that it could be widely used in wildlife species with unknown trypanosomes or mixed infections. The relatively high prevalence observed in these badgers raises the possibility that a significant proportion of UK badgers are naturally infected with trypanosomes.

Item Type: Article
Uncontrolled Keywords: 1108 Medical Microbiology, 1117 Public Health And Health Services
Subjects: Q Science > QL Zoology
R Medicine > RC Internal medicine
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: BioMed Central
Related URLs:
Date Deposited: 23 Nov 2015 10:08
Last Modified: 23 Nov 2015 10:10
DOI or Identification number: 10.1186/s13071-015-1088-7
URI: http://researchonline.ljmu.ac.uk/id/eprint/2368

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