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DEVELOPMENT OF A LIST OF REFERENCE CHEMICALS FOR EVALUATING ALTERNATIVE METHODS TO IN VIVO FISH BIOACCUMULATION TESTS

Rodriguez-Sanchez, N and Cronin, MTD and Lillicrap, A and Madden, JC and Piechota, P and Tollefsen, KE (2014) DEVELOPMENT OF A LIST OF REFERENCE CHEMICALS FOR EVALUATING ALTERNATIVE METHODS TO IN VIVO FISH BIOACCUMULATION TESTS. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY, 33 (12). ISSN 0730-7268

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Abstract

The aim to reduce the number of animals in experiments has highlighted the need to develop and validate nonanimal methods as alternatives to bioaccumulation studies using fish. The present study details a novel 3-tier approach to develop a list of reference compounds to aid this process. The approach was based on 1) the inclusion of relevant chemical classes supported by high-quality in vivo data for the bioconcentration factor (BCF), whole-body biotransformation rates (Kmet), and metabolism characterization for rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio) (tiers I and II); and 2) the refinement to ensure a broad coverage of hydrophobicity, bioconcentration potential, molecular weight, maximum molecular diameter, whole-body biotransformation half-lives, and metabolic pathways (tier III). In silico techniques were employed to predict maximal log BCF and molecular and metabolic properties. Of the 157 compounds considered as reference compounds, 144 were supported by high-quality BCF data, 8 were supported by Kmet data, and 5 were supported by in vivo metabolism data. Additional criteria for refinement of the list of reference compounds were suggested to aid practical implementation in experimental efforts. The present list of reference compounds is anticipated to facilitate the development of alternative approaches, enhance understanding of in vivo and in vitro bioaccumulation relationships, and refine in silico BCF and metabolism predictions. Environ Toxicol Chem 2014;33:2740–2752. © 2014 SETAC

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Rodriguez-Sanchez, N., Cronin, M. T. D., Lillicrap, A., Madden, J. C., Piechota, P. and Tollefsen, K. E. (2014), Development of a list of reference chemicals for evaluating alternative methods to in vivo fish bioaccumulation tests. Environ Toxicol Chem, 33: 2740–2752 which has been published in final form at http://dx.doi.org/10.1002/etc.2734. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Uncontrolled Keywords: 03 Chemical Sciences, 05 Environmental Sciences, 06 Biological Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: WILEY-BLACKWELL
Related URLs:
Date Deposited: 02 Feb 2016 14:50
Last Modified: 02 Feb 2016 14:50
DOI or Identification number: 10.1002/etc.2734
URI: http://researchonline.ljmu.ac.uk/id/eprint/2810

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