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Developmental validation of the ParaDNA(®) Intelligence System-A novel approach to DNA profiling.

Blackman, S and Dawnay, N and Ball, G and Stafford-Allen, B and Tribble, N and Rendell, P and Neary, K and Hanson, EK and Ballantyne, J and Kallifatidis, B and Mendel, J and Mills, DK and Wells, S (2015) Developmental validation of the ParaDNA(®) Intelligence System-A novel approach to DNA profiling. Forensic Science International: Genetics, 17. pp. 137-148. ISSN 1878-0326

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Abstract

DNA profiling through the analysis of STRs remains one of the most widely used tools in human identification across the world. Current laboratory STR analysis is slow, costly and requires expert users and interpretation which can lead to instances of delayed investigations or non-testing of evidence on budget grounds. The ParaDNA(®) Intelligence System has been designed to provide a simple, fast and robust way to profile DNA samples in a lab or field-deployable manner. The system analyses 5-STRs plus amelogenin to deliver a DNA profile that enables users to gain rapid investigative leads and intelligent prioritisation of samples in human identity testing applications. Utilising an innovative sample collector, minimal training is required to enable both DNA analysts and nonspecialist personnel to analyse biological samples directly, without prior processing, in approximately 75min. The test uses direct PCR with fluorescent HyBeacon(®) detection of STR allele lengths to provide a DNA profile. The developmental validation study described here followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines and tested the sensitivity, reproducibility, accuracy, inhibitor tolerance, and performance of the ParaDNA Intelligence System on a range of mock evidence items. The data collected demonstrate that the ParaDNA Intelligence System displays useful DNA profiles when sampling a variety of evidence items including blood, saliva, semen and touch DNA items indicating the potential to benefit a number of applications in fields such as forensic, military and disaster victim identification (DVI).

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 01 Mathematical Sciences, 18 Law And Legal Studies
Subjects: R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Related URLs:
Date Deposited: 05 Feb 2016 09:31
Last Modified: 06 May 2016 23:50
DOI or Identification number: 10.1016/j.fsigen.2015.04.009
URI: http://researchonline.ljmu.ac.uk/id/eprint/2830

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