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Utilization of the ability to induce activation of the nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) to assess potential cancer chemopreventive activity of liquorice samples

Basar, N and Nahar, L and Oridupa, OA and Ritchie, KJ and Talukdar, AD and Stafford, A and Kushiev, H and Kan, A and Sarker, SD (2016) Utilization of the ability to induce activation of the nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) to assess potential cancer chemopreventive activity of liquorice samples. Phytochemical Analysis, 27 (5). pp. 233-238. ISSN 1099-1565

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Abstract

Introduction – Nuclear factor (erythroid-derived 2)-like factor 2 (Nrf2) is a transcription factor that regulates expression of many detoxification enzymes. Nrf2-antioxidant responsive element (Nrf2-ARE) signalling pathway can be a target for cancer chemoprevention. Glycyrrhiza glabra, common name, ‘liquorice’, is used as a sweetening and flavouring agent, and traditionally, to treat various ailments, and implicated to chemoprevention. However, its chemopreventive property has not yet been scientifically substantiated.
Objective – To assess the ability of liquorice root samples to induce Nrf2 activation correlating to their potential chemopreventive property.
Methods – The ability of nine methanolic extracts of liquorice root samples, collected from various geographical origins, to induce Nrf2 activation was determined by the luciferase reporter assay using the ARE-reporter cell line, AREc32. The antioxidant properties were determined by the 2,2-diphenyl-1-picryhydrazyl (DPPH) and the ferric-reducing antioxidant power (FRAP) assays.
Results – All extracts exhibited free-radical-scavenging property (RC50 = 136.39-635.66 g/mL). The reducing capacity of ferrous ion was 214.46-465.59 M Fe(II)/g. Nrf2 activation indicated that all extracts induced expression of ARE-driven luciferase activity with a maximum induction of 2.3 fold relative to control. These activities varied for samples from one geographical location to another.
Conclusions – The present findings add to the existing knowledge of cancer chemoprevention by plant-derived extracts or purified phytochemicals, particularly the potential use of liquorice for this purpose.

Item Type: Article
Additional Information: This is the accepted version of the following article:Basar, N., Nahar, L., Oridupa, O. A., Ritchie, K. J., Talukdar, A. D., Stafford, A., Kushiev, H., Kan, A., and Sarker, S. D. (2016) Utilization of the Ability to Induce Activation of the Nuclear Factor (Erythroid-derived 2)-like Factor 2 (Nrf2) to Assess Potential Cancer Chemopreventive Activity of Liquorice Samples. Phytochem. Anal., 27: 233–238. doi: 10.1002/pca.2616., which has been published in final form at http://dx.doi.org/10.1002/pca.2616
Uncontrolled Keywords: 03 Chemical Sciences, 11 Medical And Health Sciences, 06 Biological Sciences
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Date Deposited: 22 Feb 2016 11:09
Last Modified: 06 Sep 2017 20:13
DOI or Identification number: 10.1002/pca.2616
URI: http://researchonline.ljmu.ac.uk/id/eprint/2973

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