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Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors

Abdellatif, AA and Zayed, G and El-Bakry, A and Zaky, A and Saleem, IY and Tawfeek, HM (2016) Novel gold nanoparticles coated with somatostatin as a potential delivery system for targeting somatostatin receptors. DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY. ISSN 0363-9045

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Abstract

Targeting of G-protein coupled receptors (GPCRs) like somatostatin-14 (SST-14) could have a potential interest in delivery of anti-cancer agents to tumor cells. Attachment of SST to different nano-carriers e.g., polymeric nanoparticles is limited due to the difficulty of interaction between SST itself and those nano-carriers. Furthermore, the instability problems associated with the final formulation. Attaching of SST to gold nanoparticles (AuNPs) using the positive and negative charge of SST and citrate-AuNPs could be considered a new technique to get stable non-aggregated AuNPs coated with SST. Different analyses techniques have been performed to proof the principle of coating between AuNPs and SST. Furthermore, cellular uptake study on HCC-1809 cell lines has been investigated to show the ability of AuNPs coated SST to enter the cells via SST receptors. Dynamic light scattering (DLS) indicated a successful coating of SST on the MUA-AuNPs surface. Furthermore, all the performed analysis including DLS, SDS-PAGE and UV-VIS absorption spectra indicated a successful coating of AuNPs with SST. Cellular uptake study on HCC-1806 cell lines showed that the number of AuNPs-SST per cell is significantly higher compared to citrate-AuNPs when quantified using inductively coupled plasma spectroscopy. Moreover, the binding of AuNPs-SST to cells can be suppressed by addition of antagonist, indicating that the binding of AuNPs-SST to cells is due to receptor-specific binding. In conclusion, AuNPs could be attached to SST via adsorption to get stable AuNPs coated SST. This new formulation has a potential to target SST receptors localized in many normal and tumor cells.

Item Type: Article
Additional Information: This is an Accepted Manuscript of an article published by Taylor & Francis in Drug Development and Industrial Pharmacy on 31 March 2016, available online: http://dx.doi.org/10.3109/03639045.2016.1173052
Uncontrolled Keywords: 1115 Pharmacology And Pharmaceutical Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: TAYLOR & FRANCIS LTD
Related URLs:
Date Deposited: 15 Apr 2016 10:14
Last Modified: 30 Mar 2017 23:51
DOI or Identification number: 10.3109/03639045.2016.1173052
URI: http://researchonline.ljmu.ac.uk/id/eprint/3433

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