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Reconstitution of muscle F-actin from Atlantic salmon (Salmo salar L.) with carotenoids – binding characteristics of astaxanthin and canthaxanthin.

Young, AJ and Pritchard, J and Lowe, GM and Buttle, L and Crampton, V (2017) Reconstitution of muscle F-actin from Atlantic salmon (Salmo salar L.) with carotenoids – binding characteristics of astaxanthin and canthaxanthin. Aquaculture Nutrition. ISSN 1365-2095

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Abstract

The binding of carotenoids to the myofibrillar protein F-actin purified from the white muscle of Atlantic salmon (Salmo salar L.) was studied using in vitro reconstitution. The binding of astaxanthin and canthaxanthin was saturable, and analysis revealed the presence of a single carotenoid-binding site. The dissociation constants (Kd) for actin prepared from 2.5 Kg FW fish were 1.04 ± 0.13 μg carotenoid mg-1 actin and 0.54 ± 0.11 μgmg-1 for astaxanthin and canthaxanthin, respectively. The saturation binding level (Bmax) for astaxanthin was 1.39 ± 0.07 μgmg-1 and 1.04 ± 0.08 μgmg-1 for canthaxanthin. These values were higher for F-actin prepared from organic and small (~0.5 Kg FW) salmon than for non-organic and larger, mature fish. The structural specificity of carotenoid binding revealed a preference for carotenoids that possess a keto group at C-4 on the end-group of the molecule, but the presence of hydroxyl groups at C-3 or C-4 reduced overall binding efficiency. The study suggests that the ability of myofibrillar proteins to bind carotenoids is not a limiting factor governing the deposition of carotenoids in the muscle of salmonids.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Young AJ, Pritchard J, Lowe GM, Crampton V, Buttle L. Reconstitution of muscle F-actin from Atlantic salmon (Salmo salar L.) with carotenoids—binding characteristics of astaxanthin and canthaxanthin. Aquacult Nutr. 2017;00:1–8. https://doi.org/10.1111/anu.12504, which has been published in final form at http://dx.doi.org/10.1111/anu.12504. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords: 0704 Fisheries Sciences
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RS Pharmacy and materia medica
S Agriculture > SH Aquaculture. Fisheries. Angling
Divisions: Natural Sciences and Psychology
Pharmacy & Biomolecular Sciences
Publisher: Wiley: 12 months
Date Deposited: 07 Sep 2016 08:59
Last Modified: 07 Sep 2017 11:08
DOI or Identification number: 10.1111/anu.12504
URI: http://researchonline.ljmu.ac.uk/id/eprint/4095

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