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The spatial distribution of the photostability of thionine in zeolite L nanochannels investigated by Photobleaching Lifetime Imaging Microscopy

Lencione, D and Gehlen, MH and Trujillo, LN and Leitao, RCF and Albuquerque, RQ (2016) The spatial distribution of the photostability of thionine in zeolite L nanochannels investigated by Photobleaching Lifetime Imaging Microscopy. Photochemical and Photobiological Sciences, 15 (3). pp. 398-404. ISSN 1474-905X

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Abstract

Dye photobleaching is a photochemical reaction that can be investigated locally using fluorescence microscopy techniques. In this study, a user-friendly computational tool to assist photobleaching experiments called Photobleaching Lifetime Imaging Microscopy (PbLIM) is presented. With this tool it is possible to recover the photobleaching kinetics spatially, where a photobleaching lifetime is generated for each pixel of the image. Our model was applied to the photobleaching process of thionine encapsulated into the one-dimensional nano-channels of Zeolite L (ZL), from where we gained insight into the molecular oxygen distribution inside the ZL channels, as well as the detailed photobleaching of the confined thionine. © The Royal Society of Chemistry and Owner Societies 2016.

Item Type: Article
Uncontrolled Keywords: 0306 Physical Chemistry (Incl. Structural), 0601 Biochemistry And Cell Biology, 0299 Other Physical Sciences
Subjects: Q Science > QD Chemistry
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Royal Society of Chemistry
Date Deposited: 29 Sep 2016 11:41
Last Modified: 18 Feb 2017 00:50
DOI or Identification number: 10.1039/c5pp00418g
URI: http://researchonline.ljmu.ac.uk/id/eprint/4163

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