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Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys.

Tsutsui, K and Otoh, M and Sakurai, K and Suzuki-Hashido, N and Hayakawa, T and Misaka, T and Ishimaru, Y and Aureli, F and Melin, AD and Kawamura, S and Imai, H (2016) Variation in ligand responses of the bitter taste receptors TAS2R1 and TAS2R4 among New World monkeys. BMC Evolutionary Biology, 16 (1). ISSN 1471-2148

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Abstract

BACKGROUND: New World monkeys (NWMs) are unique in that they exhibit remarkable interspecific variation in color vision and feeding behavior, making them an excellent model for studying sensory ecology. However, it is largely unknown whether non-visual senses co-vary with feeding ecology, especially gustation, which is expected to be indispensable in food selection. Bitter taste, which is mediated by bitter taste receptors (TAS2Rs) in the tongue, helps organisms avoid ingesting potentially toxic substances in food. In this study, we compared the ligand sensitivities of the TAS2Rs of five species of NWMs by heterologous expression in HEK293T cells and calcium imaging. RESULTS: We found that TAS2R1 and TAS2R4 orthologs differ in sensitivity among the NWM species for colchicine and camphor, respectively. We then reconstructed the ancestral receptors of NWM TAS2R1 and TAS2R4, measured the evolutionary shift in ligand sensitivity, and identified the amino acid replacement at residue 62 as responsible for the high sensitivity of marmoset TAS2R4 to colchicine. CONCLUSIONS: Our results provide a basis for understanding the differences in feeding ecology among NWMs with respect to bitter taste.

Item Type: Article
Uncontrolled Keywords: 0603 Evolutionary Biology, 0604 Genetics
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions: Natural Sciences and Psychology
Publisher: BioMed Central
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Date Deposited: 17 Oct 2016 09:13
Last Modified: 06 Sep 2017 21:37
DOI or Identification number: 10.1186/s12862-016-0783-0
URI: http://researchonline.ljmu.ac.uk/id/eprint/4620

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