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The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells.

Schrems, A and Phillips, J and Casey, DR and Wylie, D and Novakova, M and Sleytr, UB and Klug, D and Neil, MAA and Schuster, B and Ces, O (2014) The grab-and-drop protocol: a novel strategy for membrane protein isolation and reconstitution from single cells. The Analyst, 139. pp. 3296-3304. ISSN 1364-5528

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Abstract

We present a rapid and robust technique for the sampling of membrane-associated proteins from the surface of a single, live cell and their subsequent deposition onto a solid-supported lipid bilayer. As a proof of principle, this method has been used to extract green fluorescent protein (EGFP) labelled K-ras proteins located at the inner leaflet of the plasma membrane of colon carcinoma cells and to transfer them to an S-layer supported lipid bilayer system. The technique is non-destructive, meaning that both the cell and proteins are intact after the sampling operation, offering the potential for repeated measurements of the same cell of interest. This system provides the ideal tool for the investigation of cellular heterogeneity, as well as a platform for the investigation of rare cell types such as circulating tumour cells.

Item Type: Article
Uncontrolled Keywords: 0301 Analytical Chemistry, 0399 Other Chemical Sciences
Subjects: Q Science > QD Chemistry
Divisions: Built Environment
Publisher: RSC
Date Deposited: 04 Mar 2015 15:57
Last Modified: 04 Mar 2015 15:57
DOI or Identification number: 10.1039/c4an00059e
URI: http://researchonline.ljmu.ac.uk/id/eprint/536

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