Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Molecular rheometry: direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging.

Wu, Y, Stefl, M, Olzyńska, A, Hof, M, Yahioglu, G, Yip, P, Casey, DR, Ces, O, Humpolíčková, J and Kuimova, MK (2013) Molecular rheometry: direct determination of viscosity in Lo and Ld lipid phases via fluorescence lifetime imaging. Physical Chemistry Chemical Physics, 15 (36). pp. 14986-14993.

[img]
Preview
Text
PCCP Galley proofs C3CP51953H.pdf - Accepted Version

Download (1MB) | Preview

Abstract

Understanding of cellular regulatory pathways that involve lipid membranes requires the detailed knowledge of their physical state and structure. However, mapping the viscosity and diffusion in the membranes of complex composition is currently a non-trivial technical challenge. We report fluorescence lifetime spectroscopy and imaging (FLIM) of a meso-substituted BODIPY molecular rotor localised in the leaflet of model membranes of various lipid compositions. We prepare large and giant unilamellar vesicles (LUVs and GUVs) containing phosphatidylcholine (PC) lipids and demonstrate that recording the fluorescence lifetime of the rotor allows us to directly detect the viscosity of the membrane leaflet and to monitor the influence of cholesterol on membrane viscosity in binary and ternary lipid mixtures. In phase-separated 1,2-dioleoyl-sn-glycero-3-phosphocholine-cholesterol-sphingomyelin GUVs we visualise individual liquid ordered (Lo) and liquid disordered (Ld) domains using FLIM and assign specific microscopic viscosities to each domain. Our study showcases the power of FLIM with molecular rotors to image microviscosity of heterogeneous microenvironments in complex biological systems, including membrane-localised lipid rafts.

Item Type: Article
Uncontrolled Keywords: 02 Physical Sciences, 03 Chemical Sciences
Subjects: Q Science > QC Physics
Q Science > QD Chemistry
Divisions: Civil Engineering & Built Environment
Publisher: Royal Society of Chemistry
Related URLs:
Date Deposited: 04 Mar 2015 14:42
Last Modified: 04 Sep 2021 14:39
DOI or ID number: 10.1039/c3cp51953h
URI: https://researchonline.ljmu.ac.uk/id/eprint/537
View Item View Item