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Global proteome changes in the rat diaphragm induced by endurance exercise training

Sollanek, KJ and Burniston, JG and Kavazis, AN and Morton, AB and Wiggs, MP and Ahn, B and Smuder, AJ and Powers, SK (2017) Global proteome changes in the rat diaphragm induced by endurance exercise training. PLoS ONE, 12 (1). ISSN 1932-6203

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Abstract

Mechanical ventilation (MV) is a life-saving intervention for many critically ill patients. Unfor- tunately, prolonged MV results in the rapid development of diaphragmatic atrophy and weakness. Importantly, endurance exercise training results in a diaphragmatic phenotype that is protected against ventilator-induced diaphragmatic atrophy and weakness. The mechanisms responsible for this exercise-induced protection against ventilator-induced dia- phragmatic atrophy remain unknown. Therefore, to investigate exercise-induced changes in diaphragm muscle proteins, we compared the diaphragmatic proteome from sedentary and exercise-trained rats. Specifically, using label-free liquid chromatography-mass spectrome- try, we performed a proteomics analysis of both soluble proteins and mitochondrial proteins isolated from diaphragm muscle. The total number of diaphragm proteins profiled in the sol- uble protein fraction and mitochondrial protein fraction were 813 and 732, respectively. Endurance exercise training significantly (P<0.05, FDR <10%) altered the abundance of 70 proteins in the soluble diaphragm proteome and 25 proteins of the mitochondrial proteome. In particular, key cytoprotective proteins that increased in relative abundance following exer- cise training included mitochondrial fission process 1 (Mtfp1; MTP18), 3-mercaptopyruvate sulfurtransferase (3MPST), microsomal glutathione S-transferase 3 (Mgst3; GST-III), and heat shock protein 70 kDa protein 1A/1B (HSP70). While these proteins are known to be cytoprotective in several cell types, the cyto-protective roles of these proteins have yet to be fully elucidated in diaphragm muscle fibers. Based upon these important findings, future experiments can now determine which of these diaphragmatic proteins are sufficient and/or required to promote exercise-induced protection against inactivity-induced muscle atrophy.

Item Type: Article
Additional Information: Citation: Sollanek KJ, Burniston JG, Kavazis AN, Morton AB, Wiggs MP, Ahn B, et al. (2017) Global Proteome Changes in the Rat Diaphragm Induced by Endurance Exercise Training. PLoS ONE 12(1): e0171007. doi:10.1371/journal.pone.0171007
Uncontrolled Keywords: MD Multidisciplinary
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Public Library of Science (PLoS)
Date Deposited: 01 Feb 2017 16:32
Last Modified: 07 Sep 2017 19:02
DOI or Identification number: 10.1371/journal.pone.0171007
URI: http://researchonline.ljmu.ac.uk/id/eprint/5415

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