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Attenuated PGC-1 alpha Isoforms following Endurance Exercise with Blood Flow Restriction

Conceicao, MS and Traina Chacon-Mikahil, MP and Telles, GD and Libardi, CA and Mendes Junior, EM and Vechin, FC and Lugnani De Andrade, AL and Gaspari, AF and Brum, PC and Cavaglieri, CR and Serag, S and Spiegelman, BM and Hawley, JA and Camera, DM (2016) Attenuated PGC-1 alpha Isoforms following Endurance Exercise with Blood Flow Restriction. Medicine and Science in Sports and Exercise, 48 (9). pp. 1699-1707. ISSN 1530-0315

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Abstract

Introduction: Exercise performed with blood flow restriction simultaneously enhances the acute responses to both myogenic and mitochondrial pathways with roles in training adaptation. We investigated isoform-specific gene expression of the peroxisome proliferator-activated receptor gamma (PPARG) coactivator 1 and selected target genes and proteins regulating skeletal muscle training adaptation.
Methods: 9 healthy, untrained males participated in a randomized, counter-balanced, cross-over design in which each subject completed a bout of low-intensity endurance exercise performed with blood flow restriction (15 min cycling at 40% of VO2peak, BFR-EE), endurance exercise (30 min cycling at 70% of VO2peak, EE) or resistance exercise (4 x 10 repetitions of leg press at 70% of 1-repetition maximum, RE) separated by at least one week recovery. A single resting muscle biopsy (vastus lateralis) was obtained two weeks before the first exercise trial (rest) and 3 h after each boat.
Results: Total PGC-1α mRNA abundance, along with all four isoforms, increased above rest with EE only (P<0.05) being higher than BFR-EE (P<0.05). PGC-1α1, 2 and 4 were higher after EE compared to RE (P<0.05). EE also increased VEGF, Hif-1α and MuRF-1 mRNA abundance above rest (P<0.05) while COXIV mRNA expression increased with EE compared to BFR-EE (P<0.05).
Conclusion: The attenuated expression of all four PGC- 1α isoforms when endurance exercise is performed with blood flow restriction suggests this type of exercise provides an insufficient stimulus to activate the signaling pathways governing mitochondrial and angiogenesis responses observed with moderate- to high intensity endurance exercise.

Item Type: Article
Additional Information: This is a non-final version of an article published in final form in Medicine & Science in Sports & Exercise: September 2016 - Volume 48 - Issue 9 - p 1699–1707 doi: 10.1249/MSS.0000000000000970
Uncontrolled Keywords: 1106 Human Movement And Sports Science
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: American College of Sports Medicine
Related URLs:
Date Deposited: 03 Feb 2017 12:33
Last Modified: 01 Sep 2017 23:50
DOI or Identification number: 10.1249/MSS.0000000000000970
URI: http://researchonline.ljmu.ac.uk/id/eprint/5434

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