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Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections.

Castoldi, A and Herr, C and Niederstraßer, J and Labouta, HI and Melero, A and Gordon, S and Schneider-Daum, N and Bals, R and Lehr, C-M (2016) Calcifediol-loaded liposomes for local treatment of pulmonary bacterial infections. European Journal of Pharmaceutics and Biopharmaceutics. ISSN 1873-3441

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Abstract

The influence of vitamin D3 and its metabolites calcifediol (25(OH)D) and calcitriol on immune regulation and inflammation is well described, and raises the question of potential benefit against bacterial infections. In the current study, 25(OH)D was encapsulated in liposomes to enable aerosolisation, and tested for the ability to prevent pulmonary infection by Pseudomonas aeruginosa. Prepared 25(OH)D-loaded liposomes were nanosized and monodisperse, with a negative surface charge and a 25(OH)D entrapment efficiency of approximately 23%. Jet nebulisation of liposomes was seen to yield an aerosol suitable for tracheo-bronchial deposition. Interestingly, 25(OH)D in either liposomes or ethanolic solution had no effect on the release of the proinflammatory cytokine KC from Pseudomonas-infected murine epithelial cells (LA-4); treatment of infected, human bronchial 16-HBE cells with 25(OH)D liposomes however resulted in a significant reduction in bacterial survival. Together with the importance of selecting an application-appropriate in vitro model, the current study illustrates the feasibility and practicality of employing liposomes as a means to achieve 25(OH)D lung deposition. 25(OH)D-loaded liposomes further demonstrated promising effects regarding prevention of Pseudomonas infection in human bronchial epithelial cells.

Item Type: Article
Uncontrolled Keywords: 1115 Pharmacology And Pharmaceutical Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Related URLs:
Date Deposited: 05 Jun 2017 10:01
Last Modified: 12 Sep 2017 05:00
DOI or Identification number: 10.1016/j.ejpb.2016.11.026
URI: http://researchonline.ljmu.ac.uk/id/eprint/6620

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