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Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs

Menina, S and Labouta, HI and Geyer, R and Krause, T and Gordon, S and Dersch, P and Lehr, CM (2016) Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs. RSC Advances, 6. ISSN 2046-2069

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Abstract

Intracellular infections caused by invasive pathogens continue to prove difficult to combat, due in part to the commonly poor membrane permeability of anti-infective drugs. The aim of this study was to improve the intracellular delivery of one such poorly permeable (but broad-spectrum) anti-infective, gentamicin. Gentamicin was encapsulated into liposomal nanocarriers which were then surface functionalized with InvA497, a bacteria-derived invasion protein. Treatment of HEp-2 cells infected with the enteroinvasive bacteria Yersinia pseudotuberculosis or Salmonella enterica with gentamicincontaining, InvA497-functionalized liposomes resulted in a significantly greater reduction in infection load than treatment with non-functionalized liposomes, indicating that such a bacteriomimetic nanocarrier was not only able to promote successful cellular uptake of gentamicin but was also able to mediate anti-infective drug delivery to both cell cytoplasm and intracellular compartments. The developed InvA497-functionalized liposomal nanocarrier therefore holds great promise as a strategy for improving the therapy of intracellular infections.

Item Type: Article
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Royal Society of Chemistry
Date Deposited: 06 Jun 2017 08:59
Last Modified: 06 Jun 2017 08:59
DOI or Identification number: 10.1039/c6ra02988d
URI: http://researchonline.ljmu.ac.uk/id/eprint/6630

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