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The performance of a resazurin chromogenic agar plate with a combined disc method for rapid screening of extended-spectrum-β-lactamases, AmpC β-lactamases and co-β-lactamases in Enterobacteriaceae.

Teethaisong, Y and Evans, K and Nakouti, I and Tiamyom, K and Ketudat-Cairns, JR and Hobbs, G and Eumkeb, G (2017) The performance of a resazurin chromogenic agar plate with a combined disc method for rapid screening of extended-spectrum-β-lactamases, AmpC β-lactamases and co-β-lactamases in Enterobacteriaceae. Microbiology and Immunology. ISSN 0385-5600

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Abstract

A resazurin chromogenic agar (RCA) along with combined disc method has been developed as a promising method for rapid screening of extended-spectrum-β-lactamase (ESBL), AmpC β-lactamase, and co-production of ESBL and AmpC. Cefpodoxime (CPD) discs supplemented with and without clavulanic acid (CA), cloxacillin (CX), or CA+CX were evaluated against 86-molecularly confirmed β-lactamase-producing Enterobacteriaceae, including 15 ESBLs, 32 AmpCs, 9 co-producers of ESBL and AmpC, and 30 carbapenemase producers. The CA and CX synergy test successfully detected all ESBL producers (100% sensitivity and 98.6% specificity) and all AmpC producers (100% sensitivity and 96.36% specificity). This assay also exhibited a good performance in the screening for the co-existence of ESBL and AmpC (88.89% sensitivity and 100% specificity). The RCA assay is a simple and inexpensive method that allows observation of results within 7 h. It can be applicable in any microbiological laboratory, especially in the endemic areas of ESBL, AmpC, or co-β-lactamase-producing Enterobacteriaceae.

Item Type: Article
Additional Information: This is the accepted version of the following article: Teethaisong, Y., Evans, K., Nakouti, I., Tiamyom, K., Ketudat-Cairns, J. R., Hobbs, G. and Eumkeb, G. (), The performance of a resazurin chromogenic agar plate with a combined disc method for rapid screening of extended-spectrum-β-lactamases, AmpC β-lactamases and co-β-lactamases in Enterobacteriaceae. Microbiol Immunol. Accepted Author Manuscript. doi:10.1111/1348-0421.12499, which has been published in final form at http://dx.doi.org/10.1111/1348-0421.12499
Uncontrolled Keywords: 0605 Microbiology, 1107 Immunology, 1108 Medical Microbiology
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 10 Jul 2017 09:40
Last Modified: 07 Sep 2017 06:28
DOI or Identification number: 10.1111/1348-0421.12499
URI: http://researchonline.ljmu.ac.uk/id/eprint/6807

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