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Pseudomonas aeruginosa biofilm disruption using microbial surfactants.

Diaz De Rienzo, MA and Stevenson, PS and Marchant, R and Banat, IM (2016) Pseudomonas aeruginosa biofilm disruption using microbial surfactants. Journal of Applied Microbiology, 120 (4). pp. 868-876. ISSN 1364-5072

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Abstract

AIMS: To establish the ability of the rhamnolipids biosurfactants from Pseudomonas aeruginosa, in the presence and absence of caprylic acid and ascorbic acid, to disrupt bacterial biofilms, compared with the anionic alkyl sulphate surfactant Sodium dodecyl sulphate (SDS). METHODS AND RESULTS: Pseudomonas aeruginosa ATCC 15442 biofilms were disrupted by rhamnolipids at concentrations between 0·5 and 0·4 g l(-1) and with SDS at 0·8 g l(-1) . The combination of rhamnolipids 0·4 g l(-1) and caprylic acid at 0·1 g l(-1) showed a remarkable effect on biofilm disruption and cell killing. After 30 min of treatment most of the biofilm was disrupted and cell viability was significantly reduced. Neither caprylic acid nor ascorbic acid has any effect on biofilm disruption at 0·1 g l(-1) . SDS is an effective antimicrobial agent; however, in the presence of caprylic acid its effect was neutralized. CONCLUSIONS: The results show that rhamnolipids at low concentration in the presence of caprylic acid are promising molecules for inhibition/disruption of biofilms formed by Ps. aeruginosa ATCC 15442. SIGNIFICANCE AND IMPACT OF THE STUDY: The disruption of biofilms has major significance in many industrial and domestic cleaning applications and in medical situations.

Item Type: Article
Additional Information: This is the accepted version of the following article: [full citation, which has been published in final form at http://dx.doi.org/10.1111/jam.13049
Uncontrolled Keywords: MD Multidisciplinary
Subjects: Q Science > QR Microbiology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 28 Sep 2017 11:26
Last Modified: 28 Sep 2017 11:26
DOI or Identification number: 10.1111/jam.13049
URI: http://researchonline.ljmu.ac.uk/id/eprint/7238

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