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Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise.

Camera, DM and Burniston, JG and Pogson, MA and Smiles, WJ and Hawley, JA (2017) Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise. The FASEB Journal. ISSN 0892-6638

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Abstract

It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction-induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP), we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein-by-protein basis in human skeletal muscle. Age-matched, overweight males consumed 9 d of a high-fat, low-carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with 28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein-by-protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle.-Camera, D. M., Burniston, J. G., Pogson, M. A., Smiles, W. J., Hawley, J. A. Dynamic proteome profiling of individual proteins in human skeletal muscle after a high-fat diet and resistance exercise.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry And Cell Biology, 0606 Physiology, 1116 Medical Physiology
Subjects: Q Science > QM Human anatomy
Q Science > QP Physiology
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Federation of American Society of Experimental Biology
Related URLs:
Date Deposited: 10 Oct 2017 08:53
Last Modified: 10 Oct 2017 08:53
DOI or Identification number: 10.1096/fj.201700531R
URI: http://researchonline.ljmu.ac.uk/id/eprint/7309

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