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Postexercise cold water immersion modulates skeletal muscle PGC-1 alpha mRNA expression in immersed and nonimmersed limbs: evidence of systemic regulation

Allan, R and Sharples, AP and Close, GL and Drust, B and Shepherd, SO and Dutton, J and Morton, JP and Gregson, W (2017) Postexercise cold water immersion modulates skeletal muscle PGC-1 alpha mRNA expression in immersed and nonimmersed limbs: evidence of systemic regulation. Journal of Applied Physiology, 123 (2). pp. 451-459. ISSN 8750-7587

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Abstract

Mechanisms mediating postexercise cold-induced increases in PGC-1α gene expression in human skeletal muscle are yet to be fully elucidated but may involve local cooling effects on AMPK and p38 MAPK-related signaling and/or increased systemic β-adrenergic stimulation. Therefore, we aimed to examine whether postexercise cold water immersion enhancement of PGC-1α mRNA is mediated through local or systemic mechanisms. Ten subjects completed acute cycling (8 × 5 min at ~80% peak power output) followed by seated-rest (CON) or single-leg cold water immersion (CWI; 10 min, 8°C). Muscle biopsies were obtained preexercise, postexercise, and 3 h postexercise from a single limb in the CON condition but from both limbs in CWI [thereby providing tissue from a CWI and nonimmersed limb (NOT)]. Muscle temperature decreased up to 2 h postexercise following CWI (−5°C) in the immersed limb, with lesser changes observed in CON and NOT (−3°C, P < 0.05). No differences between limbs were observed in p38 MAPK phosphorylation at any time point (P < 0.05), whereas a significant interaction effect was present for AMPK phosphorylation (P = 0.031). Exercise (CON) increased gene expression of PGC-1α 3 h postexercise (~5-fold, P < 0.001). CWI augmented PGC-1α expression above CON in both the immersed (CWI; ~9-fold, P = 0.003) and NOT limbs (~12-fold, P = 0.001). Plasma normetanephrine concentration was higher in CWI vs. CON immediately postimmersion (860 vs. 665 pmol/l, P = 0.034). We report for the first time that local cooling of the immersed limb evokes transcriptional control of PGC-1α in the nonimmersed limb, suggesting increased systemic β-adrenergic activation of AMPK may mediate, in part, postexercise cold induction of PGC-1α mRNA.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 11 Medical And Health Sciences
Subjects: R Medicine > R Medicine (General)
Divisions: Sport & Exercise Sciences
Publisher: Amercian Physiological Society
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Date Deposited: 12 Oct 2017 09:47
Last Modified: 12 Oct 2017 09:47
DOI or Identification number: 10.1152/japplphysiol.00096.2017
URI: http://researchonline.ljmu.ac.uk/id/eprint/7334

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