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Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea

Edwards, T and Burke, PA and Smalley, HB and Gillies, L and Hobbs, G (2014) Loop-Mediated Isothermal Amplification Test for Detection of Neisseria gonorrhoeae in Urine Samples and Tolerance of the Assay to the Presence of Urea. Journal of Clinical Microbiology, 52 (6). pp. 6163-6165. ISSN 0095-1137

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Abstract

A loop-mediated isothermal amplification (LAMP) assay for open reading frame 1 (ORF1) of the glutamine synthetase gene of Neisseria gonorrhoeae was able to tolerate urea concentrations of ≤1.8 M, compared with a PCR assay that was functional at concentrations of <100 mM. The LAMP assay was as sensitive as the PCR assay while being faster and simpler to perform.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 07 Agricultural And Veterinary Sciences, 11 Medical And Health Sciences
Subjects: Q Science > QR Microbiology
Q Science > QR Microbiology > QR180 Immunology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: American Society for Microbiology
Related URLs:
Date Deposited: 15 Nov 2017 11:46
Last Modified: 15 Nov 2017 11:46
DOI or Identification number: 10.1128/JCM.00314-14
URI: http://researchonline.ljmu.ac.uk/id/eprint/7560

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