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A nitrocefin disc supplemented with ertapenem for rapid screening of carbapenemase-producing Enterobacteriaceae

Teethaisong, Y, Hobbs, G, Nakouti, I, Evans, K and Eumkeb, G (2018) A nitrocefin disc supplemented with ertapenem for rapid screening of carbapenemase-producing Enterobacteriaceae. Diagnostic Microbiology and Infectious Disease. ISSN 0732-8893

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Abstract

Reliable, simple and rapid methods for laboratory detection of carbapenemases are important for an appropriate antibiotic administration. A nitrocefin disc containing ertapenem for rapid screening of carbapenemase production among Enterobacteriaceae is developed in the present study. A total of 87 molecularly-confirmed Enterobacteriaceae including 31 carbapenemase producers and 56 non-carbapenemase producers, were tested with nitrocefin discs supplemented with and without ertapenem (20 μg/disc). Nitrocefin discs with ertapenem successfully discriminated all 31 carbapenemase and all non-carbapenemase producers within 30 minutes. The sensitivity and specificity of the method were 100%. The minimum inhibitory concentrations (MICs) of ertapenem against all carbapenemase-producing isolates ranged from 1 to ≥ 256 μg/mL. This simple test could help to minimize the treatment failure and control the dissemination of infections caused by carbapenemase-associated resistant bacteria. It is a promising approach that could be performed routinely in any laboratory.

Item Type: Article
Uncontrolled Keywords: 0605 Microbiology, 1103 Clinical Sciences, 1108 Medical Microbiology
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Date Deposited: 29 Jan 2018 11:31
Last Modified: 29 Jan 2018 11:31
DOI or Identification number: 10.1016/j.diagmicrobio.2017.12.023
URI: http://researchonline.ljmu.ac.uk/id/eprint/7908

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