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A comparative in situ decomposition study using still born piglets and leaf litter from a deciduous forest

Olakanye, AO, Nelson, A and Ralebitso-Senior, TK (2017) A comparative in situ decomposition study using still born piglets and leaf litter from a deciduous forest. Forensic Science International, 276. pp. 85-92. ISSN 0379-0738

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Abstract

A cadaver and dead plant organic matter, or litter, are rich energy sources that undergo a complex decomposition process, which impact the surrounding environmental microbiota. Advances in molecular microbiology techniques, with study of the 16S RNA genes, in particular, have highlighted the application of forensic ecogenomics in addressing key knowledge gaps. To investigate subsurface microbiome shifts as a novel tool to establish “postmortem microbial clock” and augment postmortem interval (PMI) and time-since-burial estimations, an in situ study with triplicate underground burials of piglets as human taphonomic proxies and Quercus robur leaf litter was monitored for 270 days. Changes in microbial community structure and composition were related directly to changes in seasonal temperature, with microbial shifts more pronounced during the summer. For example, Methylococcaceae could be used as seasonal bacterial indicators, from winter to summer, in establishing postmortem microbial clock for this site. Furthermore, Methylophilaceae (Methylophilales order) and Anaerolineaceae would differentiate for the piglet and leaf litter soils, respectively, 180 days after internment.

Item Type: Article
Uncontrolled Keywords: MD Multidisciplinary
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QR Microbiology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Related URLs:
Date Deposited: 08 Feb 2018 10:58
Last Modified: 15 Sep 2018 05:03
DOI or Identification number: 10.1016/j.forsciint.2017.04.024
URI: http://researchonline.ljmu.ac.uk/id/eprint/7969

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