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Soil metabarcoding identifies season indicators and differentiators of pig and Agrostis/Festuca spp decomposition

Olakanye, AO and Ralebitso Senior, TK Soil metabarcoding identifies season indicators and differentiators of pig and Agrostis/Festuca spp decomposition. Forensic Science International. ISSN 0379-0738 (Accepted)

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Abstract

To gain a better understanding of how environmental microbiota respond to cadaver decomposition, a forensic ecogenomic study was made with soil only control and 4 g each of S. scrofa domesticus and plant litter (Agrostis/Festuca spp) buried individually in a sandy clay loam (80 g) in sealed but perforated triplicate microcosms. The next-generation sequencing (Illumina Miseq) of the soil bacteria (16S rRNA gene) clade revealed seasonal taxomonic shifts at genus-level for the pig and plant litter microcosms compared to the non-burial controls. In particular, numerical abundances of Sphingobacterium (5.9%) and Pedobacter (24.1%) for the pig microcosms, and Rhodanobacter (18.1%) and Shinella (4.6%) for the plant litter microcosms, identified bacterial genera that could be tracked to establish a (seasonal) subsurface postmortem microbial clock. Also, family-level resolution revealed members that were unique to the control, grass and pig soils after 365 days.

Item Type: Article
Uncontrolled Keywords: MD Multidisciplinary
Subjects: Q Science > QR Microbiology
R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
Date Deposited: 10 Apr 2018 10:55
Last Modified: 10 Apr 2018 10:55
URI: http://researchonline.ljmu.ac.uk/id/eprint/8220

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