Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Acute Endurance Exercise Induces Nuclear p53 Abundance in Human Skeletal Muscle

Tachtsis, B, Smiles, WJ, Lane, SC, Hawley, JA and Camera, DM (2016) Acute Endurance Exercise Induces Nuclear p53 Abundance in Human Skeletal Muscle. Frontiers in Physiology, 7. ISSN 1664-042X

[img]
Preview
Text
Acute Endurance Exercise Induces Nuclear p53 Abundance in Human Skeletal Muscle.pdf - Published Version
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

Purpose: The tumor suppressor protein p53 may have regulatory roles in exercise response-adaptation processes such as mitochondrial biogenesis and autophagy, although its cellular location largely governs its biological role. We investigated the subcellular localization of p53 and selected signaling targets in human skeletal muscle following a single bout of endurance exercise.
Methods: Sixteen, untrained individuals were pair-matched for aerobic capacity (VO2peak) and allocated to either an exercise (EX, n = 8) or control (CON, n = 8) group. After a resting muscle biopsy, EX performed 60 min continuous cycling at ~70% of VO2peak during which time CON subjects rested. A further biopsy was obtained from both groups 3 h post-exercise (EX) or 4 h after the first biopsy (CON).
Results: Nuclear p53 increased after 3 h recovery with EX only (~48%, p < 0.05) but was unchanged in the mitochondrial or cytoplasmic fractions in either group. Autophagy protein 5 (Atg-5) decreased in the mitochondrial protein fraction 3 h post-EX (~69%, P < 0.05) but remained unchanged in CON. There was an increase in cytoplasmic levels of the mitophagy marker PINK1 following 3 h of rest in CON only (~23%, P < 0.05). There were no changes in mitochondrial, nuclear, or cytoplasmic levels of PGC-1α post-exercise in either group.
Conclusions: The selective increase in nuclear p53 abundance following endurance exercise suggests a potential pro-autophagy response to remove damaged proteins and organelles prior to initiating mitochondrial biogenesis and remodeling responses in untrained individuals.

Item Type: Article
Additional Information: Tachtsis B, Smiles WJ, Lane SC, Hawley JA and Camera DM (2016) Acute Endurance Exercise Induces Nuclear p53 Abundance in Human Skeletal Muscle. Front. Physiol. 7:144. doi: 10.3389/fphys.2016.00144
Uncontrolled Keywords: Science & Technology; Life Sciences & Biomedicine; Physiology; mitochondrial biogenesis; autophagy; mitochondrial turnover; cell signaling; skeletal muscle; HIGH-INTENSITY INTERVAL; RESISTANCE EXERCISE; MITOCHONDRIAL BIOGENESIS; AUTOPHAGY; PHOSPHORYLATION; PGC-1-ALPHA; AMPK; MODULATION; INCREASES; ADAPTATION
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Frontiers Media
Related URLs:
Date Deposited: 09 Mar 2018 11:42
Last Modified: 09 Mar 2018 11:42
DOI or Identification number: 10.3389/fphys.2016.00144
URI: http://researchonline.ljmu.ac.uk/id/eprint/8244

Actions (login required)

View Item View Item