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Rouleaux red blood cells splitting in microscopic thin blood smear images via local maxima, circles drawing, and mapping with original RBCs.

Rehman, A, Abbas, N, Saba, T, Mahmood, T and Kolivand, H (2018) Rouleaux red blood cells splitting in microscopic thin blood smear images via local maxima, circles drawing, and mapping with original RBCs. Microscopy Research and Technique. ISSN 1097-0029

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Abstract

Splitting the rouleaux RBCs from single RBCs and its further subdivision is a challenging area in computer-assisted diagnosis of blood. This phenomenon is applied in complete blood count, anemia, leukemia, and malaria tests. Several automated techniques are reported in the state of art for this task but face either under or over splitting problems. The current research presents a novel approach to split Rouleaux red blood cells (chains of RBCs) precisely, which are frequently observed in the thin blood smear images. Accordingly, this research address the rouleaux splitting problem in a realistic, efficient and automated way by considering the distance transform and local maxima of the rouleaux RBCs. Rouleaux RBCs are splitted by taking their local maxima as the centres to draw circles by mid-point circle algorithm. The resulting circles are further mapped with single RBC in Rouleaux to preserve its original shape. The results of the proposed approach on standard data set are presented and analyzed statistically by achieving an average recall of 0.059, an average precision of 0.067 and F-measure 0.063 are achieved through ground truth with visual inspection.

Item Type: Article
Uncontrolled Keywords: 0299 Other Physical Sciences, 0601 Biochemistry And Cell Biology, 0912 Materials Engineering
Subjects: Q Science > QA Mathematics > QA75 Electronic computers. Computer science
Q Science > QA Mathematics > QA76 Computer software
R Medicine > RM Therapeutics. Pharmacology
Divisions: Computer Science
Publisher: Wiley
Related URLs:
Date Deposited: 16 May 2018 11:18
Last Modified: 16 May 2018 11:18
DOI or Identification number: 10.1002/jemt.23030
URI: http://researchonline.ljmu.ac.uk/id/eprint/8510

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