Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Blood Mononuclear Cell Mitochondrial Respiratory Chain Complex IV Activity is Decreased in Multiple Sclerosis Patients: Effects of beta-Interferon Treatment

Hargreaves, IP, Mody, N, Land, J and Heales, S (2018) Blood Mononuclear Cell Mitochondrial Respiratory Chain Complex IV Activity is Decreased in Multiple Sclerosis Patients: Effects of beta-Interferon Treatment. Journal of Clinical Medicine, 7 (2). ISSN 2077-0383

[img]
Preview
Text
New paper 20.02.18.pdf - Published Version
Available under License Creative Commons Attribution.

Download (307kB) | Preview

Abstract

Objectives: Evidence of mitochondrial respiratory chain (MRC) dysfunction and oxidative stress has been implicated in the pathophysiology of multiple sclerosis (MS). However, at present, there is no reliable low invasive surrogate available to evaluate mitochondrial function in these patients. In view of the particular sensitivity of MRC complex IV to oxidative stress, the aim of this study was to assess blood mononuclear cell (BMNC) MRC complex IV activity in MS patients and compare these results to age matched controls and MS patients on β-interferon treatment.
Methods: Spectrophotometric enzyme assay was employed to measure MRC complex IV activity in blood mononuclear cell obtained multiple sclerosis patients and aged matched controls. Results: MRC Complex IV activity was found to be significantly decreased (p < 0.05) in MS patients (2.1 ± 0.8 k/nmol × 10-3; mean ± SD] when compared to the controls (7.2 ± 2.3 k/nmol × 10-3). Complex IV activity in MS patients on β-interferon (4.9 ± 1.5 k/nmol × 10-3) was not found to be significantly different from that of the controls. Conclusions: This study has indicated evidence of peripheral MRC complex IV deficiency in MS patients and has highlighted the potential utility of BMNCs as a potential means to evaluate mitochondrial function in this disorder. Furthermore, the reported improvement of complex IV activity may provide novel insights into the mode(s) of action of β- interferon.

Item Type: Article
Uncontrolled Keywords: Science & Technology; Life Sciences & Biomedicine; Medicine, General & Internal; General & Internal Medicine; mitochondrial respiratory chain; complex IV; blood mononuclear cells; multiple sclerosis; beta-Interferon; NITRIC-OXIDE; PEROXYNITRITE; DEGENERATION; DYSFUNCTION; DEFICIENCY; ASTROCYTES; DISEASE; LESIONS; DAMAGE; DNA
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: MDPI AG
Related URLs:
Date Deposited: 02 Apr 2019 08:10
Last Modified: 02 Apr 2019 08:12
DOI or Identification number: 10.3390/jcm7020036
URI: http://researchonline.ljmu.ac.uk/id/eprint/8730

Actions (login required)

View Item View Item