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Functional validation of target-site resistance mutations against sodium channel blocker insecticides (SCBIs) via molecular modeling and genome engineering in Drosophila.

Samantsidis, G-R, O'Reilly, AO, Douris, V and Vontas, J (2018) Functional validation of target-site resistance mutations against sodium channel blocker insecticides (SCBIs) via molecular modeling and genome engineering in Drosophila. Insect Biochemistry and Molecular Biology, 104. pp. 73-81. ISSN 0965-1748

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Functional validation of target-site resistance mutations against Sodium Channel Blocker Insecticides (SCBIs) via genome engineering in Drosophila.pdf - Accepted Version
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Abstract

Sodium channel blocker insecticides (SCBIs) like indoxacarb and metaflumizone offer an alternative insecticide resistance management (IRM) strategy against several pests that are resistant to other compounds. However, resistance to SCBIs has been reported in several pests, in most cases implicating metabolic resistance mechanisms, although in certain indoxacarb resistant populations of Plutella xylostella and Tuta absoluta, two mutations in the domain IV S6 segment of the voltage-gated sodium channel, F1845Y and V1848I have been identified, and have been postulated through in vitro electrophysiological studies to contribute to target-site resistance. In order to functionally validate in vivo each mutation in the absence of confounding resistance mechanisms, we have employed a CRISPR/Cas9 strategy to generate strains of Drosophila melanogaster bearing homozygous F1845Y or V1848I mutations in the para (voltage-gated sodium channel) gene. We performed toxicity bioassays of these strains compared to wild-type controls of the same genetic background. Our results indicate both mutations confer moderate resistance to indoxacarb (RR: 6-10.2), and V1848I to metaflumizone (RR: 8.4). However, F1845Y confers very strong resistance to metaflumizone (RR: >3400). Our molecular modeling studies suggest a steric hindrance mechanism may account for the resistance of both V1848I and F1845Y mutations, whereby introducing larger side chains may inhibit metaflumizone binding.

Item Type: Article
Uncontrolled Keywords: 0601 Biochemistry And Cell Biology, 0608 Zoology
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: Natural Sciences and Psychology
Publisher: Elsevier
Related URLs:
Date Deposited: 07 Jan 2019 11:10
Last Modified: 07 Jan 2019 19:05
DOI or Identification number: 10.1016/j.ibmb.2018.12.008
URI: http://researchonline.ljmu.ac.uk/id/eprint/9881

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