Facial reconstruction

Search LJMU Research Online

Browse Repository | Browse E-Theses

Four Weeks of Probiotic Supplementation Alters the Metabolic Perturbations Induced by Marathon Running: Insight from Metabolomics.

Pugh, JN, Phelan, MM, Caamaño-Gutiérrez, E, Sparks, SA, Morton, JP, Close, GL and Owens, DJ (2021) Four Weeks of Probiotic Supplementation Alters the Metabolic Perturbations Induced by Marathon Running: Insight from Metabolomics. Metabolites, 11 (8). ISSN 2218-1989

[img]
Preview
Text
Four weeks of probiotic supplementation alters the metabolic perturbations induced by marathon running Insight from metabolomics.pdf - Published Version
Available under License Creative Commons Attribution.

Download (1MB) | Preview

Abstract

Few data are available that describe how probiotics influence systemic metabolism during endurance exercise. Metabolomic profiling of endurance athletes will elucidate mechanisms by which probiotics may confer benefits to the athlete. In this study, twenty-four runners (20 male, 4 female) were block randomised into two groups using a double-blind matched-pairs design according to their most recent Marathon performance. Runners were assigned to 28-days of supplementation with a multi-strain probiotic (PRO) or a placebo (PLB). Following 28-days of supplementation, runners performed a competitive track Marathon race. Venous blood samples and muscle biopsies (vastus lateralis) were collected on the morning of the race and immediately post-race. Samples were subsequently analysed by untargeted 1H-NMR metabolomics. Principal component analysis (PCA) identified a greater difference in the post-Marathon serum metabolome in the PLB group vs. PRO. Univariate tests identified 17 non-overlapped metabolites in PLB, whereas only seven were identified in PRO. By building a PLS-DA model of two components, we revealed combinations of metabolites able to discriminate between PLB and PRO post-Marathon. PCA of muscle biopsies demonstrated no discernible difference post-Marathon between treatment groups. In conclusion, 28-days of probiotic supplementation alters the metabolic perturbations induced by a Marathon. Such findings may be related to maintaining the integrity of the gut during endurance exercise.

Item Type: Article
Uncontrolled Keywords: 0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology, 1103 Clinical Sciences
Subjects: T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: MDPI AG
Related URLs:
Date Deposited: 03 Sep 2021 11:03
Last Modified: 04 Sep 2021 05:04
DOI or ID number: 10.3390/metabo11080535
URI: https://researchonline.ljmu.ac.uk/id/eprint/15445
View Item View Item