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Photoperiod-Dependent Expression of MicroRNA in Drosophila

Pegoraro, M, Fishman, B, Zonato, V, Zouganelis, G, Francis, A, Kyriacou, CP and Tauber, E (2022) Photoperiod-Dependent Expression of MicroRNA in Drosophila. International Journal of Molecular Sciences, 23 (9). p. 4935. ISSN 1661-6596

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Like many other insects in temperate regions, Drosophila melanogaster exploits the photoperiod shortening that occurs during the autumn as an important cue to trigger a seasonal response. Flies survive the winter by entering a state of reproductive arrest (diapause), which drives the relo-cation of resources from reproduction to survival. Here, we profiled the expression of microRNA (miRNA) in long and short photoperiods and identified seven differentially expressed miRNAs (dme-mir-2b, dme-mir-11, dme-mir-34, dme-mir-274, dme-mir-184, dme-mir-184*, and dme-mir-285). Mi-sexpression of dme-mir-2b, dme-mir-184, and dme-mir-274 in pigment-dispersing, factor-expressing neurons largely disrupted the normal photoperiodic response, suggesting that these miRNAs play functional roles in photoperiodic timing. We also analyzed the targets of photoperiodic miRNA by both computational predication and by Argonaute-1-mediated immunoprecipitation of long-and short-day RNA samples. Together with global transcriptome profiling, our results expand existing data on other Drosophila species, identifying genes and pathways that are differentially regulated in different photoperiods and reproductive status. Our data suggest that post-transcriptional regulation by miRNA is an important facet of photoperiodic timing.

Item Type: Article
Uncontrolled Keywords: Animals; Drosophila; Drosophila melanogaster; MicroRNAs; Photoperiod; Diapause; Drosophila; RNA immunoprecipitation; diapause; microRNA; photoperiodism; seasonal timing; Animals; Diapause; Drosophila; Drosophila melanogaster; MicroRNAs; Photoperiod; 0399 Other Chemical Sciences; 0604 Genetics; 0699 Other Biological Sciences; Chemical Physics
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Divisions: Biological & Environmental Sciences (from Sep 19)
Publisher: MDPI AG
SWORD Depositor: A Symplectic
Date Deposited: 25 May 2022 07:56
Last Modified: 25 May 2022 08:00
DOI or ID number: 10.3390/ijms23094935
URI: https://researchonline.ljmu.ac.uk/id/eprint/16929
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