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Assessing the quantification of acetylation in konjac glucomannan via ATR-FTIR and solid-state NMR spectroscopy

Bhullar, KA, Horgan, MIM, Le, A, Fania, D, Wuhrer, R, Razmovski-Naumovski, V, Chan, K, Castignolles, P and Gaborieau, M (2022) Assessing the quantification of acetylation in konjac glucomannan via ATR-FTIR and solid-state NMR spectroscopy. Carbohydrate Polymers, 291. ISSN 0144-8617

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Dietary fiber like konjac glucomannan (KGM) is important in maintaining good human health. There is no established method for quantifying the average degree of acetylation DA of this polysaccharide. Polysaccharides are notoriously difficult to dissolve. In this study, KGM could not be fully dissolved in common solvents and was characterized in the solid state. ATR-FTIR spectroscopy enabled a fast qualitative assessment of acetylation, selective to the outer layer of KGM particles, and identifying excipients like magnesium stearate. Average DA was quantified for the first time with solid-state 13C NMR in KGM: semi-quantitative measurements on the same arbitrary scale by cross polarization (1 to 2 days) were calibrated with a few longer single-pulse excitation measurements (approximately 1 week). DA values ranged from 4 to 8% of the hexoses in the backbone, in agreement with previously reported values. This method could be used for quality control and standardization of KGM products.

Item Type: Article
Uncontrolled Keywords: Humans; Polysaccharides; Mannans; Spectroscopy, Fourier Transform Infrared; Magnetic Resonance Spectroscopy; Acetylation; Ataxia Telangiectasia Mutated Proteins; ATR-FTIR spectroscopy; Degree of acetylation; Konjac glucomannan; Quantification; Solid-state NMR spectroscopy; Solubility; Acetylation; Ataxia Telangiectasia Mutated Proteins; Humans; Magnetic Resonance Spectroscopy; Mannans; Polysaccharides; Spectroscopy, Fourier Transform Infrared; Polymers; 0303 Macromolecular and Materials Chemistry; 0305 Organic Chemistry; 0908 Food Sciences
Subjects: R Medicine > RM Therapeutics. Pharmacology
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Elsevier
SWORD Depositor: A Symplectic
Date Deposited: 12 Aug 2022 11:18
Last Modified: 01 Jun 2023 00:50
DOI or ID number: 10.1016/j.carbpol.2022.119659
URI: https://researchonline.ljmu.ac.uk/id/eprint/17381
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