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Ketoprofen and aspirin removal by laccase immobilized on date stones

Al-sareji, OJ, Meiczinger, M, Salman, JM, Al-Juboori, RA, Hashim, KS, Somogyi, V and Jakab, M (2022) Ketoprofen and aspirin removal by laccase immobilized on date stones. Chemosphere, 311 (2). ISSN 0045-6535

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In recent years, enzymatic remediation/biocatalysis has gained prominence for the bioremediation of recalcitrant chemicals. Laccase is one of the commonly investigated enzymes for bioremediation applications. There is a growing interest in immobilizing this enzyme onto adsorbents for achieving high pollutant removal through simultaneous adsorption and biodegradation. Due to the influence of the biomolecule-support interface on laccase activity and stability, it is crucial to functionalize the solid carrier prior to immobilization. Date stones (PDS),as an eco-friendly, low-cost, and effective natural adsorbent, was utilized as a carrier for laccase (fungus Trametes Versicolor). After activating PDS through chemical treatments, the surface area increased by thirty-six-fold, and carbonyl groups became more prominent. Batch experiments were carried out for ketoprofen and aspirin biodegradation in aqueous solutions. After six cycles, the
laccase maintained 54% of its original activity confirmed by oxidation tests of 2,2′-azino-bis (3 ethylbenzothiazoline-6-sulphonic acid) (ABTS). In addition, the storage, pH, and thermal stability of immobilized laccase on functionalized date stone (LFPDS) were found to be superior to that of free laccase, demonstrating its potential for ongoing applications. In the aqueous batch mode, this
immobilized laccase system was used to degrade 25 mg L-1 of ketoprofen and aspirin, resulting in almost complete removal within 4 h of treatment. This study reveals that agricultural wastes such as date stone can successfully be valorized through simple activation techniques, and the final product can be used as an adsorbent and substrate for immobilization enzyme. The high efficiency of the LFPDS in removing ketoprofen and aspirin highlights the potential of this technology for removing pharmaceuticals and merits its continued development.

Item Type: Article
Uncontrolled Keywords: Meteorology & Atmospheric Sciences; Environmental Sciences
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
R Medicine > RS Pharmacy and materia medica
S Agriculture > S Agriculture (General)
T Technology > TD Environmental technology. Sanitary engineering
Divisions: Civil Engineering & Built Environment
Publisher: Elsevier BV
SWORD Depositor: A Symplectic
Date Deposited: 07 Nov 2022 11:34
Last Modified: 06 Dec 2022 16:45
DOI or ID number: 10.1016/j.chemosphere.2022.137133
URI: https://researchonline.ljmu.ac.uk/id/eprint/18033
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