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Acute heat stress amplifies exercise‐induced metabolomic perturbations and reveals variation in circulating amino acids in endurance‐trained males

Bennett, S, Brocherie, F, Phelan, MM, Tiollier, E, Guibert, E, Morales‐Artacho, AJ, Lalire, P, Morton, JP, Louis, JB and Owens, DJ (2023) Acute heat stress amplifies exercise‐induced metabolomic perturbations and reveals variation in circulating amino acids in endurance‐trained males. Experimental Physiology. ISSN 0958-0670

Experimental Physiology - 2023 - Bennett - Acute heat stress amplifies exercise%E2%80%90induced metabolomic perturbations and.pdf - Published Version
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Open Access URL: https://doi.org/10.1113/EP090911 (Published version)


Using untargeted metabolomics, we aimed to characterise the systemic impact of environmental heat stress during exercise. Twenty-three trained male triathletes (◂◽.▸ = 64.8 ± 9.2 ml kg min−1) completed a 30-min exercise test in hot (35°C) and temperate (21°C) conditions. Venous blood samples were collected immediately pre- and post-exercise, and the serum fraction was assessed via untargeted 1H-NMR metabolomics. Data were analysed via uni- and multivariate analyses to identify differences between conditions. Mean power output was higher in temperate (231 ± 36 W) versus hot (223 ± 31 W) conditions (P < 0.001). Mean heart rate (temperate, 162 ± 10 beats min−1, hot, 167 ± 9 beats min−1, P < 0.001), peak core temperature (Trec), core temperature change (ΔTrec) (P < 0.001) and peak rating of perceived exertion (P = 0.005) were higher in hot versus temperate conditions. Change in metabolite abundance following exercise revealed distinct clustering following multivariate analysis. Six metabolites increased (2-hydroxyvaleric acid, acetate, alanine, glucarate, glucose, lactate) in hot relative to temperate (P < 0.05) conditions. Leucine and lysine decreased in both conditions but to a greater extent in temperate conditions (P < 0.05). Citrate (P = 0.04) was greater in temperate conditions whilst creatinine decreased in hot conditions only (P > 0.05). Environmental heat stress increased glycolytic metabolite abundance and led to distinct alterations in the circulating amino acid availability, including increased alanine, glutamine, leucine and isoleucine. The data highlight the need for additional exercise nutrition and metabolism research, specifically focusing on protein requirements for exercise under heat stress.

Item Type: Article
Uncontrolled Keywords: 0606 Physiology; 1106 Human Movement and Sports Sciences; 1116 Medical Physiology; Physiology
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Wiley
SWORD Depositor: A Symplectic
Date Deposited: 26 Jan 2023 10:48
Last Modified: 13 Feb 2023 09:29
DOI or ID number: 10.1113/ep090911
URI: https://researchonline.ljmu.ac.uk/id/eprint/18737
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