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Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress

Vinayavekhin, N, Wattanophas, T, Murphy, MF, Vangnai, AS and Hobbs, G (2024) Metabolomics responses and tolerance of Pseudomonas aeruginosa under acoustic vibration stress. PLoS One, 19 (1). pp. 1-19. ISSN 1932-6203

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Sound has been shown to impact microbial behaviors. However, our understanding of the chemical and molecular mechanisms underlying these microbial responses to acoustic vibration is limited. In this study, we used untargeted metabolomics analysis to investigate the effects of 100-Hz acoustic vibration on the intra- and extracellular hydrophobic metabolites of P. aeruginosa PAO1. Our findings revealed increased levels of fatty acids and their derivatives, quinolones, and N-acylethanolamines upon sound exposure, while rhamnolipids (RLs) showed decreased levels. Further quantitative real-time polymerase chain reaction experiments showed slight downregulation of the rhlA gene (1.3-fold) and upregulation of fabY (1.5-fold), fadE (1.7-fold), and pqsA (1.4-fold) genes, which are associated with RL, fatty acid, and quinolone biosynthesis. However, no alterations in the genes related to the rpoS regulators or quorum-sensing networks were observed. Supplementing sodium oleate to P. aeruginosa cultures to simulate the effects of sound resulted in increased tolerance of P. aeruginosa in the presence of sound at 48 h, suggesting a potential novel response-tolerance correlation. In contrast, adding RL, which went against the response direction, did not affect its growth. Overall, these findings provide potential implications for the control and manipulation of virulence and bacterial characteristics for medical and industrial applications.

Item Type: Article
Uncontrolled Keywords: Biofilms; Pseudomonas aeruginosa; Fatty Acids; Bacterial Proteins; Virulence Factors; Virulence; Acoustics; Vibration; Quorum Sensing; Pseudomonas aeruginosa; Vibration; Quorum Sensing; Virulence; Virulence Factors; Fatty Acids; Acoustics; Bacterial Proteins; Biofilms; General Science & Technology
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Public Library of Science
SWORD Depositor: A Symplectic
Date Deposited: 05 Apr 2024 13:25
Last Modified: 05 Apr 2024 13:30
DOI or ID number: 10.1371/journal.pone.0297030
Editors: Shastry, RP
URI: https://researchonline.ljmu.ac.uk/id/eprint/22967
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