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An exercise physiologist's guide to metabolomics

Owens, DJ and Bennett, S (2024) An exercise physiologist's guide to metabolomics. Experimental Physiology. pp. 1-14. ISSN 0958-0670

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The field of exercise physiology has undergone significant technological advancements since the pioneering works of exercise physiologists in the early to mid-20th century. Historically, the ability to detect metabolites in biofluids from exercising participants was limited to single-metabolite analyses. However, the rise of metabolomics, a discipline focused on the comprehensive analysis of metabolites within a biological system, has facilitated a more intricate understanding of metabolic pathways and networks in exercise. This review explores some of the pivotal technological and bioinformatic advancements that have propelled metabolomics to the forefront of exercise physiology research. Metabolomics offers a unique ‘fingerprint’ of cellular activity, offering a broader spectrum than traditional single-metabolite assays. Techniques, including mass spectrometry and nuclear magnetic resonance spectroscopy, have significantly improved the speed and sensitivity of metabolite analysis. Nonetheless, challenges persist, including study design and data interpretation issues. This review aims to serve as a guide for exercise physiologists to facilitate better research design, data analysis and interpretation within metabolomics. The potential of metabolomics in bridging the gap between genotype and phenotype is emphasised, underscoring the critical importance of careful study design and the selection of appropriate metabolomics techniques. Furthermore, the paper highlights the need to deeply understand the broader scientific context to discern meaningful metabolic changes. The emerging field of fluxomics, which seeks to quantify metabolic reaction rates, is also introduced as a promising avenue for future research.

Item Type: Article
Uncontrolled Keywords: exercise physiology; metabolism; metabolomics; 0606 Physiology; 1106 Human Movement and Sports Sciences; 1116 Medical Physiology; Physiology
Subjects: Q Science > QP Physiology
R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Wiley
SWORD Depositor: A Symplectic
Date Deposited: 08 Apr 2024 14:11
Last Modified: 08 Apr 2024 14:15
DOI or ID number: 10.1113/EP091059
URI: https://researchonline.ljmu.ac.uk/id/eprint/22976
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