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Conserved and species-specific transcriptional responses to daily programmed resistance exercise in rat and mouse

Viggars, MR, Sutherland, H, Cardozo, CP and Jarvis, JC (2023) Conserved and species-specific transcriptional responses to daily programmed resistance exercise in rat and mouse. The FASEB Journal, 37 (12). pp. 1-20. ISSN 0892-6638

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Mice are often used in gain or loss of function studies to understand how genes regulate metabolism and adaptation to exercise in skeletal muscle. Once-daily resistance training with electrical nerve stimulation produces hypertrophy of the dorsiflexors in rat, but not in mouse. Using implantable pulse generators, we assessed the acute transcriptional response (1-h post-exercise) after 2, 10, and 20 days of training in free-living mice and rats using identical nerve stimulation paradigms. RNA sequencing revealed strong concordance in the timecourse of many transcriptional responses in the tibialis anterior muscles of both species including responses related to “stress responses/immediate-early genes, and “collagen homeostasis,” “ribosomal subunits,” “autophagy,” and “focal adhesion.” However, pathways associated with energy metabolism including “carbon metabolism,” “oxidative phosphorylation,” “mitochondrial translation,” “propanoate metabolism,” and “valine, leucine, and isoleucine degradation” were oppositely regulated between species. These pathways were suppressed in the rat but upregulated in the mouse. Our transcriptional analysis suggests that although many pathways associated with growth show remarkable similarities between species, the absence of an actual growth response in the mouse may be because the mouse prioritizes energy metabolism, specifically the replenishment of fuel stores and intermediate metabolites.

Item Type: Article
Uncontrolled Keywords: Muscle, Skeletal; Animals; Humans; Mice; Rats; Protein Biosynthesis; Resistance Training; exercise; functional electrical stimulation; metabolism; muscle atrophy; muscle hypertrophy; transcription; Rats; Mice; Animals; Humans; Resistance Training; Protein Biosynthesis; Muscle, Skeletal; 0601 Biochemistry and Cell Biology; 0606 Physiology; 1116 Medical Physiology; Biochemistry & Molecular Biology
Subjects: R Medicine > RC Internal medicine > RC1200 Sports Medicine
Divisions: Sport & Exercise Sciences
Publisher: Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.
SWORD Depositor: A Symplectic
Date Deposited: 09 Apr 2024 14:24
Last Modified: 09 Apr 2024 14:30
DOI or ID number: 10.1096/fj.202301611R
URI: https://researchonline.ljmu.ac.uk/id/eprint/22993
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