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A novel peptide for microRNA delivery to medulloblastoma cells

Harland, E, Alhusban, D, Abdoubaki, R, Marfing, D, Simm, A, McCloskey, AP, Ehtezazi, T and Ross, K (2023) A novel peptide for microRNA delivery to medulloblastoma cells. Biomolecular Research Reports, 1 (1). pp. 1-11.

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Medulloblastoma is a paediatric brain cancer categorised into various subtypes that have differing prognostic out-comes for patients. As with other cancers, microRNAs have been implicated in medulloblastoma pathogenesis and the loss of specific miRNAs appears to contribute to the disease. There is therefore an urgent need to develop miRNA-replacement therapies for medulloblastoma. However, methods for targeted delivery of small RNAs to medulloblastoma cells have not been fully established. As a step towards tackling this challenge, we have developed self-assembling peptide nanoparticles for small RNA delivery to medulloblastoma cells. We generated an amphiphilic peptide, TY-28, using solid-phase peptide synthesis and combined TY-28 with miR-124-3p. Analysis of the resulting complexes by electron microscopy and dynamic light scattering confirmed the formation of nanoparticles. The ability of the NPs to penetrate cells was monitored by labelling the miRNA with a fluorescent dye. The NP:miRNA complexes were readily internalised by group 3 medulloblastoma cells, and the accumulation of the complexes increased over time. Levels of uptake were 6-fold higher at 24 hours compared to 4 hours in serum-free medium. The uptake of the NPs complexes by the cells did not differ in the presence and absence of serum, suggesting the presence of serum did not affect complex stability. Our findings point to the translational potential of self-assembled NPs to delivery miR-NA mimics to medulloblastoma cells.

Item Type: Article
Uncontrolled Keywords: Medulloblastoma; microRNA; Cancer; Peptides; Nanoparticles
Subjects: Q Science > QH Natural history > QH301 Biology
R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Liverpool John Moores University
SWORD Depositor: A Symplectic
Date Deposited: 03 Jul 2024 12:20
Last Modified: 03 Jul 2024 12:20
URI: https://researchonline.ljmu.ac.uk/id/eprint/23688
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