Potential Effects of Pseuderanthemum palatiferum Extract on Inhibiting Adipogenesis and Promoting Lipolysis in 3T3-L1 Cell Line

Nuallaong, A, Dunkhunthod, B, Lowe, G ORCID: 0000-0001-7566-7166, Thipart, K and Hengpratom, T (2026) Potential Effects of Pseuderanthemum palatiferum Extract on Inhibiting Adipogenesis and Promoting Lipolysis in 3T3-L1 Cell Line. Trends in Sciences, 23 (4). p. 11840. ISSN 1686-3933

Preview

Text Potential_Effects_of_Pseuderanthemum_palatiferum_E.pdf - Published Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview

Abstract

Obesity is a global health concern driven by excessive lipid accumulation and adipocyte dysfunction, requires therapeutic strategies that both inhibit fat formation and promote lipid degradation. This study investigated the dual anti-adipogenic and pro-lipolytic effects of Pseuderanthemum palatiferum leaf extract (PPE) in 3T3-L1 adipocytes. The phytochemical analysis of PPE performed by using gas chromatography-mass spectrometry (GC-MS) and Liquid chromatography-mass spectrometry (LC-MS). The findings revealed that GC-MS analysis of PPE identified 35 volatile compounds, with benzofuran (29.94%) as the major constituent. LC-MS detected 27 non-volatiles phytochemicals, including chlorogenic acid and p-coumaric acid. PPE exhibited no significant cytotoxic effects on 3T3-L1 preadipocytes at doses ranging from 50-150 μg/mL. At 150 μg/mL, PPE reduced lipid accumulation by 37.84% and increased glycerol release by 60.46%, indicating both an anti-adipogenic and a lipolytic properties. Gene expression analysis revealed that pre-treatment with 150 μg/mL of PPE promoted the upregulation of early adipogenic markers (C/EBPα, ACC1, FAS, and GLUT4) while downregulating markers associated with mature adipocytes (Adiponectin, AP2, and CD36), indicating interference with adipocyte differentiation and lipid synthesis. Additionally, PPE lowered intracellular reactive oxygen species, suggesting an antioxidative contribution to its anti-obesity action. Collectively, this study provides the first evidence that P. palatiferum exerts complementary regulation of adipogenesis and lipolysis in 3T3-L1 cells, offering new insight into its multi-target mechanisms and potential as a natural therapeutic candidate for obesity management.

Item Type:	Article
Uncontrolled Keywords:	3205 Medical Biochemistry and Metabolomics; 31 Biological Sciences; 32 Biomedical and Clinical Sciences; Diabetes; Nutrition; Complementary and Integrative Health; Obesity; 5.1 Pharmaceuticals
Subjects:	R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Walailak University
Date of acceptance:	24 October 2025
Date of first compliant Open Access:	29 May 2026
Date Deposited:	29 May 2026 13:10
Last Modified:	29 May 2026 13:10
DOI or ID number:	10.48048/tis.2026.11840
URI:	https://researchonline.ljmu.ac.uk/id/eprint/28680

View Item