Protective role of hydroalcoholic extract of Medusomyces gisevii L. in non-alcoholic fatty liver disease: insights from a murine model

Gashti, RZ, Karami, M, Habibi, E ORCID: 0000-0002-7367-1484, Abbasi, A, Parsay, S, Modanloo, M, Khalili, HA and Shokrzadeh, M (2025) Protective role of hydroalcoholic extract of Medusomyces gisevii L. in non-alcoholic fatty liver disease: insights from a murine model. Research in Pharmaceutical Sciences, 21 (1). pp. 77-95. ISSN 1735-5362

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Abstract

Background and purpose: This study explored the impact of the hydroalcoholic extract of Medusomyces gisevii L. (HEMG), a promising source for dietary use, on NAFLD in male mice.

Experimental approach: The essential oil of MG was characterized using GC-MS and the HEMG, obtained through continuous maceration. Male albino mice were subjected to 7 groups (n = 9), including normal diet, high-fat diet (HFD, 12 weeks), HEMG (62.5, 125, 250, and 500 mg/kg, orally, 8 weeks), or vitamin E (20 mg/kg, orally, 8 weeks) supplementation with HFD. Blood samples were analyzed for serum biomarkers, and liver mitochondria were isolated to assess oxidative stress markers. Histopathological examinations of liver tissue were conducted.

Findings/Results: MG was rich in cyclohexanol, carvacrol, and phenol, with HEMG exhibiting an antioxidant activity of 50.14 ± 3.56 μg/mL. It contained 73.47 ± 0.85 mg of gallic acid equivalents per g of TPC and 62.56 ± 1.30 mg/g of TTC, indicating the significant antioxidant properties of HEMG. Mice on an HFD exhibited elevated serum biomarkers, including ALT, AST, ALP, TG, TC, and LDL, along with a reduction in HDL levels. Oxidative stress factors, including ROS, protein carbonyl, and MDA, increased, while mitochondrial function, GSH, catalase, and SOD were decreased in the NAFLD groups. Furthermore, treatment with HEMG supplementation led to improvements in serum biomarkers and enhanced oxidative stress markers, thus alleviating liver damage and hepatic steatosis caused by the HFD.

Conclusion and implications: These results suggest that HEMG holds promise as a candidate in addressing NAFLD.

Item Type:	Article
Uncontrolled Keywords:	Kombucha; Liver enzymes; <italic>Medusomyces gisevii</italic>; Non-alcoholic fatty liver disease; Oxidative stress; Vitamin E; Kombucha; Liver enzymes; Medusomyces gisevii; Non-alcoholic fatty liver disease; Oxidative stress; Vitamin E; 3214 Pharmacology and Pharmaceutical Sciences; 32 Biomedical and Clinical Sciences; Dietary Supplements; Liver Disease; Digestive Diseases; Prevention; Nutrition; Complementary and Integrative Health; Chronic Liver Disease and Cirrhosis; 1115 Pharmacology and Pharmaceutical Sciences; 3214 Pharmacology and pharmaceutical sciences
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Biological and Environmental Sciences (from Sep 19)
Publisher:	Wolters Kluwer Health
Date of acceptance:	22 September 2025
Date of first compliant Open Access:	1 June 2026
Date Deposited:	01 Jun 2026 11:22
Last Modified:	01 Jun 2026 11:22
DOI or ID number:	10.4103/RPS.RPS_121_25
URI:	https://researchonline.ljmu.ac.uk/id/eprint/28691

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