Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics

Jacobsen, MD, Beynon, RJ ORCID: 0000-0003-0857-495X, Gethings, LA, Claydon, AJ, Langridge, JI, Vissers, JPC, Brown, AJP and Hammond, DE ORCID: 0000-0002-6326-8739 (2018) Specificity of the osmotic stress response in Candida albicans highlighted by quantitative proteomics. Scientific Reports, 8 (1). ISSN 2045-2322

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Abstract

Stress adaptation is critical for the survival of microbes in dynamic environments, and in particular, for fungal pathogens to survive in and colonise host niches. Proteomic analyses have the potential to significantly enhance our understanding of these adaptive responses by providing insight into post-transcriptional regulatory mechanisms that contribute to the outputs, as well as testing presumptions about the regulation of protein levels based on transcript profiling. Here, we used label-free, quantitative mass spectrometry to re-examine the response of the major fungal pathogen of humans, Candida albicans, to osmotic stress. Of the 1,262 proteins that were identified, 84 were down-regulated in response to 1M NaCl, reflecting the decrease in ribosome biogenesis and translation that often accompanies stress. The 64 up-regulated proteins included central metabolic enzymes required for glycerol synthesis, a key osmolyte for this yeast, as well as proteins with functions during stress. These data reinforce the view that adaptation to salt stress involves a transient reduction in ribosome biogenesis and translation together with the accumulation of the osmolyte, glycerol. The specificity of the response to salt stress is highlighted by the small proportion of quantified C. albicans proteins (5%) whose relative elevated abundances were statistically significant.

Item Type:	Article
Uncontrolled Keywords:	Humans; Candida albicans; Fungal Proteins; Proteomics; Gene Expression Regulation, Fungal; Osmotic Pressure; Candida albicans; Fungal Proteins; Gene Expression Regulation, Fungal; Humans; Osmotic Pressure; Proteomics; 3107 Microbiology; 3101 Biochemistry and Cell Biology; 32 Biomedical and Clinical Sciences; 31 Biological Sciences; Biotechnology; Infectious Diseases; 2.2 Factors relating to the physical environment; Infection; Candida albicans; Fungal Proteins; Gene Expression Regulation, Fungal; Humans; Osmotic Pressure; Proteomics
Subjects:	Q Science > QH Natural history > QH301 Biology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	Springer
Date of acceptance:	13 September 2018
Date of first compliant Open Access:	7 July 2026
Date Deposited:	07 Jul 2026 12:23
Last Modified:	07 Jul 2026 12:23
DOI or ID number:	10.1038/s41598-018-32792-6
URI:	https://researchonline.ljmu.ac.uk/id/eprint/28972

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