Small-RNA sequencing reveals altered skeletal muscle microRNAs and snoRNAs signatures in weanling male offspring from mouse dams fed a low protein diet during lactation

Kanakis, I, Alameddine, M, Folkes, L, Moxon, S, Myrtziou, I and Ozanne, SE (2021) Small-RNA sequencing reveals altered skeletal muscle microRNAs and snoRNAs signatures in weanling male offspring from mouse dams fed a low protein diet during lactation. Cells, 10 (5). ISSN 2073-4409

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Abstract

Maternal diet during gestation and lactation affects the development of skeletal muscles in offspring and determines muscle health in later life. In this paper, we describe the association between maternal low protein diet-induced changes in offspring skeletal muscle and the differential expression (DE) of small non-coding RNAs (sncRNAs). We used a mouse model of maternal protein restriction, where dams were fed either a normal (N, 20%) or a low protein (L, 8%) diet during gestation and newborns were cross-fostered to N or L lactating dams, resulting in the generation of NN, NL and LN offspring groups. Total body and tibialis anterior (TA) weights were decreased in weanling NL male offspring but were not different in the LN group, as compared to NN. However, histological evaluation of TA muscle revealed reduced muscle fibre size in both groups at weaning. Small RNA-sequencing demonstrated DE of multiple miRs, snoRNAs and snRNAs. Bioinformatic analyses of miRs-15a, -34a, -122 and -199a, in combination with known myomiRs, confirmed their implication in key muscle-specific biological processes. This is the first comprehensive report for the DE of sncRNAs in nutrition-associated programming of skeletal muscle development, highlighting the need for further research to unravel the detailed molecular mechanisms.

Item Type:	Article
Uncontrolled Keywords:	maternal protein restriction; muscle development; offspring; microRNAs; sncRNAs; snoRNAs; Muscle, Skeletal; Animals; Mice; Bacterial Proteins; Luminescent Proteins; MicroRNAs; RNA, Small Nucleolar; Diet, Protein-Restricted; Sequence Analysis, DNA; Computational Biology; Weaning; Muscle Development; Lactation; Female; Male; Maternal Nutritional Physiological Phenomena; RNA, Small Untranslated; maternal protein restriction; microRNAs; muscle development; offspring; sncRNAs; snoRNAs; Animals; Bacterial Proteins; Computational Biology; Diet, Protein-Restricted; Female; Lactation; Luminescent Proteins; Male; Maternal Nutritional Physiological Phenomena; Mice; MicroRNAs; Muscle Development; Muscle, Skeletal; RNA, Small Nucleolar; RNA, Small Untranslated; Sequence Analysis, DNA; Weaning; 3215 Reproductive Medicine; 32 Biomedical and Clinical Sciences; Human Genome; Nutrition; Genetics; Biotechnology; Breastfeeding, Lactation and Breast Milk; Women's Health; Pediatric Research Initiative; 1.1 Normal biological development and functioning; 4.1 Discovery and preclinical testing of markers and technologies; Musculoskeletal; Reproductive health and childbirth; 2 Zero Hunger; Animals; Bacterial Proteins; Computational Biology; Diet, Protein-Restricted; Female; Lactation; Luminescent Proteins; Male; Maternal Nutritional Physiological Phenomena; Mice; MicroRNAs; Muscle Development; Muscle, Skeletal; RNA, Small Nucleolar; RNA, Small Untranslated; Sequence Analysis, DNA; Weaning; 31 Biological sciences; 32 Biomedical and clinical sciences
Subjects:	Q Science > QL Zoology
Divisions:	Pharmacy and Biomolecular Sciences
Publisher:	MDPI AG
Date of acceptance:	9 May 2021
Date of first compliant Open Access:	9 October 2026
Date Deposited:	09 Oct 2026 15:15
Last Modified:	09 Oct 2026 15:15
DOI or ID number:	10.3390/cells10051166
URI:	https://researchonline.ljmu.ac.uk/id/eprint/29646

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