Khan, KM, Nahar, L, Evans, AR, Ismail, FMD, Dempster, NM, Al-Groshi, A, Zazoianu, AG, Wansi, JD and Sarker, SD Evaluation of cytotoxicity of Trillium govanianum (Melanthiaceae). Phytotherapy Research. ISSN 1099-1573 (Accepted)

Text Cytotoxicity of the Roots of Trillium govanianum Against Breast (MCF7), Liver (HepG2), Lung (A549) and Urinary Bladder (EJ138) Carcinoma Cells .pdf - Accepted Version Restricted to Repository staff only Download (413kB)

Abstract

Trillium govanianum Wall. (Melanthiaceae alt. Trilliaceae), commonly known as “nagchhatry” or “teen patra”, is a native species of the Himalayas. It is used in various traditional medicines containing both steroids and sex hormones. In folk medicine, the rhizomes of T. govanianum are used to treat boils, dysentery, inflammation, menstrual and sexual disorders, as an antiseptic and in wound-healing. With the only exception of the recent report on the isolation of a new steroidal saponin, govanoside A, together with three known steroidal compounds with antifungal property from this plant, there has been no systematic pharmacological and phytochemical work performed on T. govanianum. This paper reports, for the first time, on the cytotoxicity of the methanol (MeOH) extract of the roots of T. govanianum and its solid-phase extraction (SPE) fractions against four human carcinoma cell lines: breast (MCF7), liver (HEPG2), lung (A549) and urinary bladder (EJ138), using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) cytotoxicity assay, and LC-ESI-QTOF-MS analysis of the SPE fractions. The MeOH extract and all SPE fractions exhibited considerable levels of cytotoxicity against all cell lines with the IC50 values ranging between 5-16 μg/mL. Like other Trillium species, presence of

Item Type:	Article
Uncontrolled Keywords:	11 Medical And Health Sciences, 03 Chemical Sciences, 06 Biological Sciences
Subjects:	R Medicine > RS Pharmacy and materia medica
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Wiley
Date Deposited:	06 Jun 2016 09:09
Last Modified:	04 Sep 2021 12:51
URI:	https://researchonline.ljmu.ac.uk/id/eprint/3712

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