Menina, S, Labouta, HI, Geyer, R, Krause, T, Gordon, S, Dersch, P and Lehr, CM (2016) Invasin-functionalized liposome nanocarriers improve the intracellular delivery of anti-infective drugs. RSC Advances, 6. ISSN 2046-2069

	Text Menina et al 2016.pdf - Published Version Available under License Creative Commons Attribution. Download (417kB)
	Text Menina et al 2016 Supplementary Information.pdf - Supplemental Material Available under License Creative Commons Attribution. Download (694kB)

Abstract

Intracellular infections caused by invasive pathogens continue to prove difficult to combat, due in part to the commonly poor membrane permeability of anti-infective drugs. The aim of this study was to improve the intracellular delivery of one such poorly permeable (but broad-spectrum) anti-infective, gentamicin. Gentamicin was encapsulated into liposomal nanocarriers which were then surface functionalized with InvA497, a bacteria-derived invasion protein. Treatment of HEp-2 cells infected with the enteroinvasive bacteria Yersinia pseudotuberculosis or Salmonella enterica with gentamicincontaining, InvA497-functionalized liposomes resulted in a significantly greater reduction in infection load than treatment with non-functionalized liposomes, indicating that such a bacteriomimetic nanocarrier was not only able to promote successful cellular uptake of gentamicin but was also able to mediate anti-infective drug delivery to both cell cytoplasm and intracellular compartments. The developed InvA497-functionalized liposomal nanocarrier therefore holds great promise as a strategy for improving the therapy of intracellular infections.

Item Type:	Article
Subjects:	R Medicine > RM Therapeutics. Pharmacology
Divisions:	Pharmacy & Biomolecular Sciences
Publisher:	Royal Society of Chemistry
Date Deposited:	06 Jun 2017 08:59
Last Modified:	04 Sep 2021 03:56
DOI or Identification number:	10.1039/c6ra02988d
URI:	https://researchonline.ljmu.ac.uk/id/eprint/6630

Actions (login required)

View Item