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Dynamin function is important for chemokine receptor-induced cell migration.

Jacques, RO and Mills, SC and Cazzonatto Zerwes, P and Fagade, FO and Green, JE and Downham, S and Sexton, DW and Mueller, A (2015) Dynamin function is important for chemokine receptor-induced cell migration. Cell Biochemistry and Function, 33 (6). pp. 407-414. ISSN 1099-0844

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Abstract

The HIV viral entry co-receptors CCR5 and CXCR4 function physiologically as typical chemokine receptors. Activation leads to cytosolic signal transduction that results in a variety of cellular responses such as cytoskeletal rearrangement and chemotaxis (CTX). Our aim was to investigate the signalling pathways involved in CC and CXC receptor-mediated cell migration. Inhibition of dynamin I and II GTPase with dynasore completely inhibited CCL3-stimulated CTX in THP-1 cells, whereas the dynasore analogue Dyngo-4a, which is a more potent inhibitor, showed reduced ability to inhibit CC chemokine-induced CTX. In contrast, dynasore was not able to block cell migration via CXCR4. The same activation/inhibition pattern was verified in activated T lymphocytes for different CC and CXC chemokines. Cell migration induced by CC and CXC receptors does not rely on active internalization processes driven by dynamin because the blockade of internalization does not affect migration, but it might rely on dynamin interaction with the cytoskeleton. We identify here a functional difference in how CC and CXC receptor migration is controlled, suggesting that specific signalling networks are being employed for different receptor classes and potentially specific therapeutic targets to prevent receptor migration can be identified. Copyright © 2015 John Wiley & Sons, Ltd.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: "Dynamin function is important for chemokine receptor-induced cell migration", which has been published in final form at http://dx.doi.org/10.1002/cbf.3131. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords: 0601 Biochemistry And Cell Biology
Subjects: R Medicine > RS Pharmacy and materia medica
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
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Date Deposited: 05 Nov 2015 10:41
Last Modified: 01 Aug 2016 23:50
DOI or Identification number: 10.1002/cbf.3131
URI: http://researchonline.ljmu.ac.uk/id/eprint/2079

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