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A Systems Based Investigation into Vitamin D and Skeletal Muscle Repair, Regeneration and Hypertrophy

Owens, DJ, Sharples, AP, Polydorou, I, Alwan, N, Donovan, TD, Tang, J, Fraser, WD, Cooper, RG, Morton, JP, Stewart, CE and Close, GL (2015) A Systems Based Investigation into Vitamin D and Skeletal Muscle Repair, Regeneration and Hypertrophy. American Journal of Physiology Endocrinology and Metabolism, 309 (12). E1019-E1031. ISSN 0193-1849

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Abstract

Skeletal muscle is a direct target for vitamin D. Observational studies suggest that low 25[OH] D correlates with functional recovery of skeletal muscle following eccentric contractions in humans and crush injury in rats. However, a definitive association is yet to be established. To address this gap in knowledge in relation to damage repair, a randomised, placebo-controlled trial was performed in 20 males with insufficient concentrations of serum 25(OH) D (45 +/- 25 nmol/l). Prior to and following 6 wk of supplemental vitamin D-3 (4,000 IU/day) or placebo (50 mg of cellulose), participants performed 20 x10 damaging eccentric contractions of the knee extensors, with peak torque measured over the following 7 days of recovery. Parallel experimentation using isolated human skeletal muscle-derived myoblast cells from biopsies of 14 males with low serum 25(OH) D (37 +/- 11 nmol/l) were subjected to mechanical wound injury, which enabled corresponding in vitro studies of muscle repair, regeneration, and hypertrophy in the presence and absence of 10 or 100 nmol 1 alpha,25(OH)(2)D-3. Supplemental vitamin D-3 increased serum 25(OH) D and improved recovery of peak torque at 48 h and 7 days postexercise. In vitro, 10 nmol 1 alpha,25(OH)(2)D-3 improved muscle cell migration dynamics and resulted in improvedmyotube fusion/differentiation at the biochemical, morphological, and molecular level together with increased myotube hypertrophy at 7 and 10 days postdamage. Together, these preliminary data are the first to characterize a role for vitamin D in human skeletal muscle regeneration and suggest that maintaining serum 25(OH) D may be beneficial for enhancing reparative processes and potentially for facilitating subsequent hypertrophy.

Item Type: Article
Uncontrolled Keywords: 06 Biological Sciences, 11 Medical And Health Sciences
Subjects: Q Science > QP Physiology
Divisions: Sport & Exercise Sciences
Publisher: American Psychological Society
Date Deposited: 26 Oct 2015 15:10
Last Modified: 29 Jan 2016 11:30
URI: http://researchonline.ljmu.ac.uk/id/eprint/2240

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