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Applicability of the ParaDNA(®) Screening System to Seminal Samples.

Tribble, ND and Miller, JA and Dawnay, N and Duxbury, NJ (2015) Applicability of the ParaDNA(®) Screening System to Seminal Samples. Journal of Forensic Sciences, 60 (3). pp. 690-692. ISSN 0022-1198

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Abstract

Seminal fluid represents a common biological material recovered from sexual assault crime scenes. Such samples can be prescreened using different techniques to determine cell type and relative amount before submitting for full STR profiling. The ParaDNA(®) Screening System is a novel forensic test which identifies the presence of DNA through amplification and detection of two common STR loci (D16S539 and TH01) and the Amelogenin marker. The detection of the Y allele in samples could provide a useful tool in the triage and submission of sexual assault samples by enforcement authorities. Male template material was detected on a range of common sexual assault evidence items including cotton pillow cases, condoms, swab heads and glass surfaces and shows a detection limit of 1 in 1000 dilution of neat semen. These data indicate this technology has the potential to be a useful tool for the detection of male donor DNA in sexual assault casework.

Item Type: Article
Additional Information: This is the peer reviewed version of the following article: Tribble, N. D., Miller, J. A.D., Dawnay, N. and Duxbury, N. J. (2015), Applicability of the ParaDNA® Screening System to Seminal Samples. J Forensic Sci, 60: 690–692 which has been published in final form at http:..dx.doi.org/10.1111/1556-4029.12742]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords: 0399 Other Chemical Sciences, 0699 Other Biological Sciences, 1103 Clinical Sciences
Subjects: H Social Sciences > HV Social pathology. Social and public welfare. Criminology
Q Science > QH Natural history > QH301 Biology
R Medicine > RA Public aspects of medicine > RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine
Divisions: Pharmacy & Biomolecular Sciences
Publisher: Wiley
Related URLs:
Date Deposited: 08 Feb 2016 09:25
Last Modified: 29 Apr 2016 23:50
DOI or Identification number: 10.1111/1556-4029.12742
URI: http://researchonline.ljmu.ac.uk/id/eprint/2848

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