Storm, MP and Sorrell, I and Shipley, R and Regan, S and Luetchford, KA and Sathish, J and Webb, SD and Ellis, MJ (2016) Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture. Journal of Visualized Experiments, 111 (e53431). pp. 1-12. ISSN 1940-087X
jove-protocol-53431-hollow-fiber-bioreactors-for-in-vivo-like-mammalian-tissue-culture.pdf - Published Version
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Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic. More recently, there is a growing body of data demonstrating more in vivo-like behaviors of cells grown in 3-dimensional culture systems. This manuscript describes in detail the set-up and operation of a hollow fiber bioreactor system for the in vivo-like culture of mammalian cells. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. The system is designed to fit onto the shelf of a standard CO2 incubator and is simple enough to be set-up by any competent cell biologist with a good understanding of aseptic technique. The systems utility is demonstrated by culturing the hepatocarcinoma cell line HepG2/C3A for 7 days. Further to this and in line with other published reports on the functionality of cells grown in 3-dimensional culture systems the cells are shown to possess increased albumin production (an important hepatic function) when compared to standard 2-dimensional tissue culture.
|Subjects:||Q Science > QH Natural history > QH301 Biology|
|Publisher:||Journal of Visualized Experiments|
|Date Deposited:||10 Jun 2016 11:36|
|Last Modified:||07 Oct 2016 11:10|
|DOI or Identification number:||10.3791/53431|
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